Huang Fei, Du Xinyi, Ma Zongjie, Liu Guiqin, Wang Changfa, Zhou Miaomiao
College of Agriculture and Biology, Liaocheng Research Institute of Donkey High-Efficiency Breeding and Ecological Feeding, Liaocheng University, Liaocheng 252000, China.
Animals (Basel). 2024 Oct 19;14(20):3027. doi: 10.3390/ani14203027.
This study investigated the effects of adding methionine (Met) in milk production and the milk and blood metabolites of lactating donkeys. Eighteen healthy multiparous donkeys in early-stage lactation were selected for this study. The donkeys were randomly divided into three groups and fed diets with different levels of Met: control group (C, Met 0 g/d), Met group I (M1, Met 5 g/d), and Met group II (M2, Met 15 g/d). The total duration of the experiment was 5 weeks. Donkey milk and blood samples were collected at the end of the experiment. The milk yield and composition, milk, and serum metabolites were analyzed. The results showed that the addition of 5 g of Met significantly increased milk yield as well as the milk composition contents of protein, fat, lactoferrin, polyunsaturated fatty acid (PFA), solids, and solids-not-fat (SNF) ( < 0.05). Significant differences in metabolites were detected among the different samples of milk ( < 0.05). The addition of Met increased the levels of milk metabolites, such as myristic acid, d-glutamine, l-aspartic acid, and LPS 16:0. A total of 753 metabolites were detected in the serum, including 17 differential metabolites between C and M1 and 48 differential metabolites between C and M2. The levels of serum metabolites, such as l-ascorbate, inositol, and l-lysine, were up-regulated by the addition of Met ( < 0.05). The above results indicated that Met increased donkey milk production and milk composition yield and improved milk metabolites by regulating serum metabolites. These results provide a foundation for improving the nutritional needs of lactating donkeys and the nutritional regulation of donkey milk synthesis.
本研究调查了添加蛋氨酸（Met）对泌乳期母驴产奶量以及乳汁和血液代谢产物的影响。本研究选取了18头处于泌乳早期的健康经产母驴。将这些母驴随机分为三组，分别饲喂不同蛋氨酸水平的日粮：对照组（C，Met 0 g/d）、蛋氨酸组I（M1，Met 5 g/d）和蛋氨酸组II（M2，Met 15 g/d）。实验总时长为5周。在实验结束时采集母驴乳汁和血液样本。分析了产奶量及成分、乳汁和血清代谢产物。结果表明，添加5 g蛋氨酸显著提高了产奶量以及乳汁中蛋白质、脂肪、乳铁蛋白、多不饱和脂肪酸（PFA）、固体成分和非脂固体（SNF）的含量（<0.05）。在不同乳汁样本中检测到代谢产物存在显著差异（<0.05）。添加蛋氨酸增加了乳汁代谢产物的水平，如肉豆蔻酸、D-谷氨酰胺、L-天冬氨酸和LPS 16:0。血清中共检测到753种代谢产物，其中C组和M1组之间有17种差异代谢产物，C组和M2组之间有48种差异代谢产物。添加蛋氨酸上调了血清代谢产物的水平，如L-抗坏血酸、肌醇和L-赖氨酸（<0.05）。上述结果表明，蛋氨酸通过调节血清代谢产物提高了母驴的产奶量和乳汁成分产量，并改善了乳汁代谢产物。这些结果为改善泌乳期母驴的营养需求和驴奶合成的营养调控提供了依据。
