Tong Manman, Li Shuyi, Hui Fang, Meng Fanzhu, Li Li, Shi Binlin, Zhao Yanli, Guo Xiaoyu, Guo Yongmei, Yan Sumei
Inner Mongolia Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Antioxidants (Basel). 2024 Feb 24;13(3):275. doi: 10.3390/antiox13030275.
Donkey milk is a traditional medicinal food with various biological activities. However, its production is very low, and lactating donkeys often experience oxidative stress, leading to a further decline in milk yield. In this study, we supplemented the diets of lactating donkeys with yeast selenium (SY) to investigate its effects on lactation performance, antioxidant status, and immune responses, and we expected to determine the optimum additive level of SY in the diet. For this study, 28 healthy lactating Dezhou donkeys with days in milk (DIM, 39.93 ± 7.02 d), estimated milk yield (EMY, 3.60 ± 0.84 kg/d), and parity (2.82 ± 0.48) were selected and randomly divided into 4 groups of 7 donkeys in each: Group SY-0 (control), Group SY-0.15, Group SY-0.3, and Group SY-0.5, with selenium supplementation of 0, 0.15, 0.3, and 0.5 mg of Se/kg DM (in form of SY) to the basal diet, respectively. The results showed a dose-dependent increase in milk yield, milk component yield, milk protein production efficiency, milk production efficiency, the activities of glutathione peroxidases (GSH-Px), catalase (CAT), and total antioxidant capacity (T-AOC), as well as the content of serum interleukin-10 (IL-10), white blood cells (WBC), lymphocytes (LYM), red blood cells (RBC), hematocrit, plasma selenium, and milk selenium. Conversely, it presented a dose-dependent decrease in the activity of nitric oxide synthase (iNOS) and the contents of malondialdehyde (MDA), reactive oxygen species (ROS), nitric oxide (NO), interleukin-1β (IL-1β), interleukin-6 (IL-6), and interferon-γ (IFN-γ). In conclusion, the results confirmed that dietary supplementation with SY can improve lactation performance, antioxidant status, and immune responses in lactating donkeys, and the recommended dose of SY was 0.3 mg/kg.
驴奶是一种具有多种生物活性的传统药用食品。然而，其产量极低，且泌乳母驴常经历氧化应激，导致产奶量进一步下降。在本研究中，我们在泌乳母驴的日粮中添加酵母硒（SY），以研究其对泌乳性能、抗氧化状态和免疫反应的影响，并期望确定日粮中SY的最佳添加水平。本研究选取28头健康的德州泌乳母驴，泌乳天数（DIM，39.93±7.02天）、估计产奶量（EMY，3.60±0.84千克/天）和胎次（2.82±0.48），随机分为4组，每组7头：SY-0组（对照组）、SY-0.15组、SY-0.3组和SY-0.5组，分别在基础日粮中添加0、0.15、0.3和0.5毫克硒/千克干物质（以SY形式）。结果表明，产奶量、乳成分产量、乳蛋白生产效率、产奶效率、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）活性和总抗氧化能力（T-AOC）以及血清白细胞介素-10（IL-10）、白细胞（WBC）、淋巴细胞（LYM）、红细胞（RBC）、血细胞比容、血浆硒和乳硒含量呈剂量依赖性增加。相反，一氧化氮合酶（iNOS）活性以及丙二醛（MDA）、活性氧（ROS）、一氧化氮（NO）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和干扰素-γ（IFN-γ）含量呈剂量依赖性降低。总之，结果证实日粮添加SY可改善泌乳母驴的泌乳性能、抗氧化状态和免疫反应，SY的推荐剂量为0.3毫克/千克。
