Novotny M, Jemiolo B, Harvey S, Wiesler D, Marchlewska-Koj A
Science. 1986 Feb 14;231(4739):722-5. doi: 10.1126/science.3945805.
While assessing a potential role of adrenal glands in the production of the hitherto unidentified puberty-delaying pheromone of female mice, the urinary volatile profiles of normal and adrenalectomized animals were quantitatively compared. Six components, whose concentrations were depressed after adrenalectomy, were identified: 2-heptanone, trans-5-hepten-2-one, trans-4-hepten-2-one, n-pentyl acetate, cis-2-penten-1-yl acetate, and 2,5-dimethylpyrazine. When these laboratory-synthesized chemicals were added (in their natural concentrations) to either previously inactive urine from adrenalectomized females or plain water, the biological activity was fully restored.
在评估肾上腺在雌性小鼠迄今尚未确定的青春期延迟信息素产生中的潜在作用时，对正常和肾上腺切除动物的尿液挥发性成分进行了定量比较。确定了六种成分，肾上腺切除后其浓度降低：2-庚酮、反式-5-庚烯-2-酮、反式-4-庚烯-2-酮、乙酸正戊酯、顺式-2-戊烯-1-基乙酸酯和2,5-二甲基吡嗪。当将这些实验室合成的化学物质（以其天然浓度）添加到肾上腺切除雌性动物先前无活性的尿液或纯水中时，生物活性完全恢复。
