Ma W, Miao Z, Novotny M V
Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
Biol Reprod. 1998 Dec;59(6):1317-20. doi: 10.1095/biolreprod59.6.1317.
Mature female mice, grouped in the absence of a male stimulus, exhibit a suppressed estrous cycle (the so-called Lee-Boot effect). We have designed a series of experiments to elucidate the involvement of the adrenal gland in this phenomenon. Our initial results indicate that adrenalectomized mice exhibit a regular estrous cycle in either isolated or grouped conditions. A single, intact mouse caged with five adrenalectomized females showed repeated normal cycles. When the urine samples from group-caged intact mice or group-caged adrenalectomized mice were applied to the external nares of singly caged females, estrous cycles were inhibited in the animals receiving urine from the intact mice but not from the adrenalectomized mice. In addition, corticosterone therapy restored the function of estrus suppression in grouped, adrenalectomized mice. We had previously shown that the urinary excretion of several volatile compounds (2-heptanone, trans-5-hepten-2-one, trans-4-hepten-2-one, pentyl acetate, cis-2-penten-1-yl acetate, and 2,5-dimethylpyrazine) was adrenal mediated (Science 1986; 231:722-725). A further testing of these compounds in relation to estrus suppression has now revealed that a mixture of these compounds is effective, but removing 2, 5-dimethylpyrazine from the mixture abolished the biological response. The overall results of this study show conclusively an important role of the adrenal gland and adrenal-mediated urinary metabolites in estrus suppression.
在没有雄性刺激的情况下分组饲养的成年雌性小鼠，其发情周期受到抑制（即所谓的李-布特效应）。我们设计了一系列实验来阐明肾上腺在这一现象中的作用。我们的初步结果表明，肾上腺切除的小鼠在单独饲养或分组饲养条件下都表现出规律的发情周期。一只完整的小鼠与五只肾上腺切除的雌性小鼠关在一个笼子里，其发情周期会反复正常。当将分组饲养的完整小鼠或分组饲养的肾上腺切除小鼠的尿液样本涂抹在单独饲养的雌性小鼠的外鼻孔时，接受完整小鼠尿液的动物的发情周期受到抑制，而接受肾上腺切除小鼠尿液的动物则不受影响。此外，皮质酮治疗可恢复分组饲养的肾上腺切除小鼠的发情抑制功能。我们之前已经表明，几种挥发性化合物（2-庚酮、反式-5-庚烯-2-酮、反式-4-庚烯-2-酮、乙酸戊酯、顺式-2-戊烯-1-基乙酸酯和2,5-二甲基吡嗪）的尿液排泄是由肾上腺介导的（《科学》1986年；231:722 - 725）。现在对这些化合物与发情抑制相关的进一步测试表明，这些化合物的混合物是有效的，但从混合物中去除2,5-二甲基吡嗪会消除生物学反应。这项研究的总体结果确凿地表明了肾上腺和肾上腺介导的尿液代谢产物在发情抑制中的重要作用。
