Stolfa Stefania, Caggiano Giuseppina, Ronga Luigi, Dalfino Lidia, Centrone Francesca, Sallustio Anna, Sacco Davide, Mosca Adriana, Stufano Monica, Saracino Annalisa, De Gennaro Nicolo', Casulli Daniele, Netti Nicola, Soldano Savino, Faggiano Maria, Loconsole Daniela, Tafuri Silvio, Grasso Salvatore, Chironna Maria
Microbiology and Virology Unit, Department of Interdisciplinary Medicine, University of Bari "A. Moro", 70124 Bari, Italy.
Hygiene Section, Department of Interdisciplinary Medicine, University of Bari "A. Moro", 70124 Bari, Italy.
Microorganisms. 2024 Sep 27;12(10):1962. doi: 10.3390/microorganisms12101962.
is an emerging yeast considered a serious threat to global health. We report the first case of candidemia in Southern Italy, characterized using whole genome sequencing (WGS), and compared with a second strain isolated from a patient who presented as -colonized following screening. The strain was isolated from clinical samples, identified via MALDI-TOF, and subjected to WGS. Antifungal susceptibility testing was performed using commercial broth microdilution plates, and resistance protein sequences were evaluated with TBLASTN-2.15.0. Following the initial isolation from patient A, active surveillance and environmental investigations were implemented for all ICU patients. Of the 26 ICU surfaces sampled, 46.1% tested positive for via real-time PCR. Screening identified a second patient (patient B) as -colonized. The phylogenetic characterization of strains from patients A and B, based on the D1/D2 region of the 28s rDNA and the internal transcribed spacer (ITS) region, showed high similarity with strains from Lebanon. SNP analysis revealed high clonality, assigning both strains to clade I, indicating a significant similarity with Lebanese strains. This case confirms the alarming spread of infections and highlights the need for stringent infection control measures to manage outbreaks.
是一种新兴的酵母菌，被认为对全球健康构成严重威胁。我们报告了意大利南部首例念珠菌血症病例，通过全基因组测序（WGS）进行特征分析，并与从一名筛查后呈定植状态的患者分离出的第二株菌株进行比较。该菌株从临床样本中分离出来，通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）鉴定，并进行全基因组测序。使用商业肉汤微量稀释板进行抗真菌药敏试验，并用TBLASTN-2.15.0评估耐药蛋白序列。从患者A最初分离出该菌株后，对所有重症监护病房（ICU）患者实施了主动监测和环境调查。在采集的26个ICU表面样本中，46.1%通过实时聚合酶链反应（PCR）检测呈阳性。筛查确定第二名患者（患者B）为定植状态。基于28s核糖体DNA（rDNA）的D1/D2区域和内部转录间隔区（ITS）区域，对患者A和B的菌株进行系统发育特征分析，结果显示与黎巴嫩的菌株高度相似。单核苷酸多态性（SNP）分析显示高度克隆性，将两株菌株都归为进化枝I，表明与黎巴嫩菌株有显著相似性。该病例证实了感染的惊人传播，并强调需要采取严格的感染控制措施来管理疫情爆发。
