Panneitz Ana Karolina, Braga Eduarda Ribeiro, Petri Fernando Antonio Moreira, Menegatt Jean Carlo Olivo, Driemeier David, Maes Dominiek, Oliveira Luís Guilherme de
Swine Medicine Laboratory, School of Agricultural and Veterinary Sciences, São Paulo State University (Unesp), Jaboticabal 14884-900, Brazil.
Veterinary Pathology Department, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre 91540-000, Brazil.
Microorganisms. 2024 Sep 30;12(10):1988. doi: 10.3390/microorganisms12101988.
() is the key pathogen of the porcine respiratory disease complex (PRDC) and contributes to pleurisy in pigs. Due to its limited metabolism and laborious cultivation, molecular tools are useful for diagnosis. This study investigated the genetic diversity of in slaughter pigs with pneumonia and pleurisy, and it assessed co-infections by type A (PM), (APP), and swine influenza virus A (sIVA). Lungs ( = 70) with different pleurisy scores and lesions compatible with infection were collected for convenience. Macroscopic and microscopic evaluations were performed. was detected using qPCR, and MLST was used for genetic characterization. Co-infections with PM and APP were also evaluated by qPCR, while the immunohistochemistry assessed sIVA infection. All lungs were positive for . Histopathology confirmed -associated lesions. MLST characterization was possible in 25 lungs and revealed 10 distinct allelic profiles, with none matching known sequence types in the public database. Co-infections were detected in 40% of the samples with APP and 32% with PM, with 12% showing both pathogens and 52% of the samples presenting microscopic lesions compatible with sIVA infection. The diverse genetic profiles found underscore the need for research on isolation and potential pathogenic variations.
（）是猪呼吸道疾病综合征（PRDC）的关键病原体，可导致猪胸膜炎。由于其代谢有限且培养费力，分子工具对诊断很有用。本研究调查了患有肺炎和胸膜炎的屠宰猪中（）的遗传多样性，并评估了A型胸膜肺炎放线杆菌（PM）、副猪嗜血杆菌（APP）和甲型猪流感病毒（sIVA）的共感染情况。为方便起见，收集了具有不同胸膜炎评分且有与（）感染相符病变的肺脏（n = 70）。进行了宏观和微观评估。使用qPCR检测（），并使用多位点序列分型（MLST）进行遗传特征分析。还通过qPCR评估了与PM和APP的共感染情况，而免疫组织化学评估了sIVA感染。所有肺脏（）检测均呈阳性。组织病理学证实了与（）相关的病变。在25个肺脏中进行了MLST特征分析，发现了10种不同的等位基因谱，没有一个与公共数据库中已知的序列类型匹配。在40%的样本中检测到与APP的共感染，32%的样本检测到与PM的共感染，12%的样本同时显示两种病原体，52%的样本呈现与sIVA感染相符的微观病变。发现的多样遗传谱强调了对分离和潜在致病变异进行研究的必要性。
