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从核糖体DNA操纵子中学习：对该群体的重新分析。

Learning from the rDNA Operon: A Reanalysis of the Group.

作者信息

Corsaro Daniele

机构信息

CHLAREAS, 54500 Vandoeuvre-lès-Nancy, France.

出版信息

Microorganisms. 2024 Oct 21;12(10):2105. doi: 10.3390/microorganisms12102105.

DOI:10.3390/microorganisms12102105
PMID:39458413
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11510093/
Abstract

The molecular classification of is currently based on the analysis of 18S rDNA sequences, delimiting around twenty genotypes (T1-T23). In some cases, however, the resolution of 18S is limited, and other genetic markers could be useful for unravelling poorly resolved lineages. In this study, the partial large subunit (LSU) of rDNA and ITS were used to re-examine the group (T2/T6 lineage), which consists of various poorly defined lineages, including the T2 and T6 genotypes. New sequences overlapping 18S, ITS, and LSU were recovered. The analysis placed previously identified partial ITS-LSU sequences as T2/T6 and further confirmed the separation of the OX1 lineage from T2. In addition, analysis of the second internal transcribed spacer (ITS-2) suggests that multiple species may be present within the T6 and OX1 lineages. The results obtained from the T2/T6 lineage analysis confirm the utility of partial LSU and ITS for the study of , suggesting their advantage for disentangling complex lineages.

摘要

目前的分子分类是基于对18S rDNA序列的分析，划定了大约二十种基因型（T1 - T23）。然而，在某些情况下，18S的分辨率有限，其他遗传标记可能有助于解析分辨率较低的谱系。在本研究中，rDNA的部分大亚基（LSU）和ITS被用于重新研究该类群（T2/T6谱系），该谱系由各种定义不明确的谱系组成，包括T2和T6基因型。回收了与18S、ITS和LSU重叠的新序列。分析将先前鉴定的部分ITS - LSU序列归类为T2/T6，并进一步证实了OX1谱系与T2的分离。此外，对第二内部转录间隔区（ITS - 2）的分析表明，T6和OX1谱系中可能存在多个物种。从T2/T6谱系分析中获得的结果证实了部分LSU和ITS在该研究中的实用性，表明它们在解析复杂谱系方面的优势。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2639/11510093/637f9ef00c06/microorganisms-12-02105-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2639/11510093/4af514c39323/microorganisms-12-02105-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2639/11510093/88cd704c8d69/microorganisms-12-02105-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2639/11510093/4149e49f6833/microorganisms-12-02105-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2639/11510093/882d26e93404/microorganisms-12-02105-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2639/11510093/00f38020434a/microorganisms-12-02105-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2639/11510093/637f9ef00c06/microorganisms-12-02105-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2639/11510093/4af514c39323/microorganisms-12-02105-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2639/11510093/88cd704c8d69/microorganisms-12-02105-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2639/11510093/4149e49f6833/microorganisms-12-02105-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2639/11510093/882d26e93404/microorganisms-12-02105-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2639/11510093/00f38020434a/microorganisms-12-02105-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2639/11510093/637f9ef00c06/microorganisms-12-02105-g006.jpg

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