Kim Y H, Ock M S, Yun H C, Hwang M Y, Yu H S, Kong H H, Chung D I
Department of Parasitology, Kyungpook National University School of Medicine, Taegu, Korea.
Korean J Parasitol. 1996 Dec;34(4):259-66. doi: 10.3347/kjp.1996.34.4.259.
The taxonomic validity of morphological group III Acanthamoeba spp. is uncertain. In the present study, six type strains of group III Acanthamoeba spp., A. culbertsoni, A. healyi, A. pustulosa, A. palestinensis, A. royreba and A. lenticulata were subjected for the evaluation of their taxonomic validity by comparison of the isoenzyme patterns by isoelectic focusing on polyacrylamide gels, mitochondrial DNA (Mt DNA) restriction fragment length polymorphism (RFLP), and small subunit ribosomal DNA (ssu rDNA) PCR-RFLP patterns. The Mt DNA RFLP patterns were heterogeneous between the species. The type strains of A. palestinensis and A. pustulosa showed almost identical patterns of isoenzymes and rDNA PCR RFLP with an estimated sequence divergence of 2.6%. The other species showed heterogeneous patterns of isoenzymes and rDNA PCR-RFLP. It is likely that A. pustulosa is closely related with A. palestinensis and that the former may be regarded as a junior synonym of the latter.
形态学分类的III组棘阿米巴属物种的分类学有效性尚不确定。在本研究中，通过在聚丙烯酰胺凝胶上进行等电聚焦比较同工酶模式、线粒体DNA（Mt DNA）限制性片段长度多态性（RFLP）以及小亚基核糖体DNA（ssu rDNA）PCR-RFLP模式，对III组棘阿米巴属的六个模式菌株，即库氏棘阿米巴、希氏棘阿米巴、脓疱棘阿米巴、巴勒斯坦棘阿米巴、罗伊雷巴棘阿米巴和透镜状棘阿米巴进行了分类学有效性评估。物种之间的Mt DNA RFLP模式存在异质性。巴勒斯坦棘阿米巴和脓疱棘阿米巴的模式菌株显示出几乎相同的同工酶和rDNA PCR RFLP模式，估计序列差异为2.6%。其他物种显示出异质的同工酶和rDNA PCR-RFLP模式。脓疱棘阿米巴可能与巴勒斯坦棘阿米巴密切相关，前者可能被视为后者的次异名。
