Yamada Hideto, Shimada Shigeki, Ota Hajime, Kobayashi Yuta, Fukushi Yoshiyuki, Wada Shinichiro, Kataoka Soromon
Center for Recurrent Pregnancy Loss, Teine Keijinkai Hospital, Sapporo 006-8555, Hokkaido, Japan.
Department of Obstetrics and Gynecology, Teine Keijinkai Hospital, Sapporo 006-8555, Hokkaido, Japan.
Microorganisms. 2024 Oct 21;12(10):2110. doi: 10.3390/microorganisms12102110.
This prospective cohort study aimed to evaluate the performance of Flora select™ (FS), a newly developed real-time PCR test, for the assessment of the vaginal microbiome during early pregnancy. Five hundred and fifty-six pregnant women underwent examinations of FS, Nugent score-a Gram-staining scoring system for the diagnosis of bacterial vaginosis (BV)-and conventional bacterial culture between 8 weeks and 12 gestational weeks. Nugent scores of 0-3, 4-6, and ≥7 were found in 469 (84.2%), 41 (7.4%), and 47 (8.5%) of the women, respectively. Relative dominance rates of species of high (≥80% medium (50%≤, <80%), and low (0.1≤, <50%), and no detection (<0.1%) were 63.0%, 8.8%, 17.1%, and 11.2%, respectively. , , , , , and species were detected in 23.9%, 17.6%, 17.1%, 7.0%, 23.0%, and 4.9% of the women, respectively. species were detected in all women with Nugent scores ≥7 and were detected in 40.4% of them. BV-associated bacterial species were also detected in 70.7% of women with Nugent scores of 4-6. , , , , , and species were highly prevalent in women with Nugent scores ≥4 or species <50%. FS detected , , and species more effectively than conventional bacterial culture. FS could determine relative dominance rates of species in the vaginal microbiome, and simultaneously detect four kinds of BV-associated bacteria, and species. Therefore, FS may be clinically useful for the screening of the vaginal microbiome during pregnancy to prevent preterm birth and for the assessment of the vaginal microbiome after BV treatments.
这项前瞻性队列研究旨在评估新开发的实时荧光定量聚合酶链反应检测法Flora select™（FS）在孕早期评估阴道微生物群的性能。556名孕妇在妊娠8周和12周之间接受了FS检测、用于诊断细菌性阴道病（BV）的革兰氏染色评分系统Nugent评分以及传统细菌培养。分别有469名（84.2%）、41名（7.4%）和47名（8.5%）女性的Nugent评分为0 - 3、4 - 6和≥7。高（≥80%）、中（50%≤，<80%）、低（0.1≤，<50%）和未检测到（<0.1%）的菌种相对优势率分别为63.0%、8.8%、17.1%和11.2%。分别有23.9%、17.6%、17.1%、7.0%、23.0%和4.9%的女性检测到了 、 、 、 、 和 菌种。在所有Nugent评分≥7的女性中均检测到了 菌种，其中40.4%的女性检测到了 菌种。在Nugent评分为4 - 6的女性中，70.7%也检测到了与BV相关的细菌菌种。 、 、 、 、 和 菌种在Nugent评分≥4或 菌种<50%的女性中高度流行。FS比传统细菌培养更有效地检测到了 、 和 菌种。FS可以确定阴道微生物群中 菌种的相对优势率，同时检测四种与BV相关的细菌，即 和 菌种。因此，FS在临床上可能有助于孕期阴道微生物群的筛查以预防早产，以及BV治疗后阴道微生物群的评估。
