揭示代谢综合征中的脂质组学改变：饮食诱导的大鼠模型中血浆、肝脏和脂肪组织的研究。

Unveiling Lipidomic Alterations in Metabolic Syndrome: A Study of Plasma, Liver, and Adipose Tissues in a Dietary-Induced Rat Model.

机构信息

Group for Nutritional Biochemistry and Dietology, Centre of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia.

Laboratory of Forensic Medicine & Toxicology, Department of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.

出版信息

Nutrients. 2024 Oct 13;16(20):3466. doi: 10.3390/nu16203466.

DOI:10.3390/nu16203466

PMID:39458462

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11509917/

Abstract

Metabolic syndrome (MetS) is a complex condition characterized by fat accumulation, dyslipidemia, impaired glucose control and hypertension. In this study, rats were fed a high-fat high-fructose (HFF) diet in order to develop MetS. After ten weeks, the dietary-induced MetS was confirmed by higher body fat percentage, lower HDL-cholesterol and increased blood pressure in the HFF-fed rats compared to the normal-fed control animals. However, the effect of MetS development on the lipidomic signature of the dietary-challenged rats remains to be investigated. To reveal the contribution of specific lipids to the development of MetS, the lipid profiling of rat tissues particularly susceptible to MetS was performed using untargeted UHPLC-QTOF-MS/MS lipidomic analysis. A total of 37 lipid species (mainly phospholipids, triglycerides, sphingolipids, cholesterol esters, and diglycerides) in plasma, 43 lipid species in liver, and 11 lipid species in adipose tissue were identified as dysregulated between the control and MetS groups. Changes in the lipid signature of selected tissues additionally revealed systemic changes in the dietary-induced rat model of MetS.

摘要

代谢综合征（MetS）是一种以脂肪堆积、血脂异常、葡萄糖控制受损和高血压为特征的复杂病症。在这项研究中，大鼠被喂食高脂肪高果糖（HFF）饮食，以发展代谢综合征。十周后，与正常饮食对照动物相比，HFF 喂养的大鼠体脂肪百分比更高、高密度脂蛋白胆固醇（HDL-cholesterol）更低、血压升高，证实了饮食诱导的代谢综合征的发生。然而，代谢综合征的发展对饮食挑战大鼠脂质组学特征的影响仍有待研究。为了揭示特定脂质对代谢综合征发展的贡献，使用非靶向 UHPLC-QTOF-MS/MS 脂质组学分析对特别容易受到代谢综合征影响的大鼠组织的脂质谱进行了分析。在血浆中鉴定出 37 种脂质（主要是磷脂、甘油三酯、鞘脂、胆固醇酯和二甘油酯）、肝中 43 种脂质和脂肪组织中 11 种脂质在对照组和代谢综合征组之间存在差异。对选定组织的脂质特征变化的进一步研究揭示了饮食诱导的代谢综合征大鼠模型中的全身性变化。