The Absence of Phasins PhbP2 and PhbP3 in Determines the Growth and Poly-3-hydroxybutyrate Synthesis.

Phasins are proteins located on the surface of poly-3-hydroxybutyrate (P3HB) granules that affect the metabolism of the polymer, the size and number of the granules, and some also have stress-protecting and growth-promoting effects. This study evaluated the effect of inactivating two new phasins (PhbP2 or PhbP3) on the cellular growth, production, and molecular mass of P3HB in cultures under low or high oxygen transfer rates (OTR). The results revealed that under high OTRₘₐₓ conditions (between 8.1 and 8.9 mmol L h), the absence of phasins PhbP2 and PhbP3 resulted in a strong negative effect on the growth rate; in contrast, the rates of specific oxygen consumption increased in both cases. This behavior was not observed under a low oxygen transfer rate (3.9 ± 0.71 mol L h), where cellular growth and oxygen consumption were the same for the different strains evaluated. It was observed that at high OTR, the absence of PhbP3 affected the production of P3HB, decreasing it by 30% at the end of cultivation. In contrast, the molecular weight remained constant over time. In summary, the absence of phasin PhbP3 significantly impacted the growth rate and polymer synthesis, particularly at high maximum oxygen transfer rates (OTRₘₐₓ).