Hu Shijun, Wang Meizhen, Yan Xiaohui, Cheng Xiaomao
Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming 650224, China.
Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China.
Plants (Basel). 2024 Oct 11;13(20):2851. doi: 10.3390/plants13202851.
Genetic diversity is crucial for determining the evolutionary potential of a species and is essential for developing optimal conservation strategies. The impact of habitat fragmentation on the genetic diversity of food-deceptive orchids seems to be unpredictable because of their specialized seed and pollen dispersal mechanisms. The habitat of deceptive was severely fragmented during the past half century. This study investigated the genetic diversity and structure of seven fragmented populations in Shangrila County using AFLP markers. A total of 376 alleles were identified, with a range of 70 to 81 alleles per locus. The species exhibited considerable genetic diversity, as evidenced by an average Nei's gene diversity () of 0.339 and a Shannon's information index () of 0.505, with all loci being polymorphic. Based on Molecular Variance (AMOVA), 8.75% of the genetic differentiation was found among populations, while the remaining 91.25% of genetic variation occurred within populations. Population structure analysis revealed that the germplasm can be categorized into 2 distinct groups, among which there was significant gene flow. Despite habitat fragmentation, still retained a high level of genetic diversity, and the substantial gene flow (5.0826) is a key factor in maintaining the genetic diversity. These findings offer valuable insights for the conservation and potential use of genetic resources.
遗传多样性对于确定一个物种的进化潜力至关重要，对于制定最佳保护策略也必不可少。由于其特殊的种子和花粉传播机制，生境破碎化对食源性欺骗性兰花遗传多样性的影响似乎难以预测。在过去的半个世纪里，欺骗性兰花的栖息地严重破碎化。本研究使用AFLP标记调查了香格里拉县七个破碎化种群的遗传多样性和结构。共鉴定出376个等位基因，每个位点的等位基因范围为70至81个。该物种表现出相当高的遗传多样性，平均奈氏基因多样性（）为0.339，香农信息指数（）为0.505，所有位点均为多态性。基于分子方差分析（AMOVA），发现种群间8.75%的遗传分化，而其余91.25%的遗传变异发生在种群内。种群结构分析表明，该兰花种质可分为2个不同的组，其中存在显著的基因流。尽管生境破碎化，该兰花仍保留了高水平的遗传多样性，大量的基因流（5.0826）是维持遗传多样性的关键因素。这些发现为该兰花遗传资源的保护和潜在利用提供了有价值的见解。
