Moya-Cuevas José, Ortiz-Gutiérrez Elizabeth, López-Sánchez Patricio, Simón-Moya Miguel, Ballester Patricia, Álvarez-Buylla Elena R, Ferrándiz Cristina
Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia, 46022 Valencia, Spain.
Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Campus de Teatinos, 29071 Málaga, Spain.
Plants (Basel). 2024 Oct 18;13(20):2927. doi: 10.3390/plants13202927.
Different convergent evolutionary strategies adopted by angiosperm fruits lead to diverse functional seed dispersal units. Dry dehiscent fruits are a common type of fruit, characterized by their lack of fleshy pericarp and the release of seeds at maturity through openings (dehiscence zones, DZs) in their structure. In previous decades, a set of core players in DZ formation have been intensively characterized in Arabidopsis and integrated in a gene regulatory network (GRN) that explains the morphogenesis of these tissues. In this work, we compile all the experimental data available to date to build a discrete Boolean model as a mechanistic approach to validate the network and, if needed, to identify missing components of the GRN and/or propose new hypothetical regulatory interactions, but also to provide a new formal framework to feed further work in Brassicaceae fruit development and the evolution of seed dispersal mechanisms. Hence, by means of exhaustive in-silico validations and experimental evidence, we are able to incorporate both the NO TRANSMITTING TRACT (NTT) transcription factor as a new additional node, and a new set of regulatory hypothetical rules to uncover the dynamics of Arabidopsis DZ specification.
被子植物果实采用的不同趋同进化策略导致了功能多样的种子传播单元。干果是一种常见的果实类型，其特点是没有肉质果皮，成熟时通过结构上的开口（开裂区，DZs）释放种子。在过去几十年里，拟南芥中一组参与开裂区形成的核心因子已得到深入研究，并整合到一个基因调控网络（GRN）中，该网络解释了这些组织的形态发生。在这项工作中，我们汇总了迄今为止所有可用的实验数据，构建了一个离散布尔模型，作为一种机械方法来验证该网络，并在需要时识别GRN中缺失的成分和/或提出新的假设调控相互作用，同时也提供一个新的形式框架，为十字花科果实发育和种子传播机制的进化研究提供进一步的思路。因此，通过详尽的计算机模拟验证和实验证据，我们能够将无传输道（NTT）转录因子作为一个新的额外节点纳入，并提出一组新的假设调控规则，以揭示拟南芥开裂区特化的动态过程。
