Kaya Madalina Georgiana Albu, Simonca Alice Geanina, Rau Ileana, Coman Alina Elena, Marin Minodora Maria, Popa Lacramioara, Trusca Roxana, Dinu-Pirvu Cristina-Elena, Ghica Mihaela Violeta
Department of Collagen, National Research and Development Institute for Textiles and Leather-Division of Leather and Footwear Research Institute, 93 Ion Minulescu Str., 031215 Bucharest, Romania.
Faculty of Chemical Engineering and Biotechnology, National University of Science and Technology Politehnica Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania.
Pharmaceuticals (Basel). 2024 Oct 7;17(10):1336. doi: 10.3390/ph17101336.
It is well known that periodontitis affects the gums and surrounding connective tissue. The chronic inflammatory response induced by bacteria in the gingival tissue leads to the loss of the collagen connection between the tooth and the bone and ultimately to bone loss.
In this context, the aim of this research was the obtaining and characterization of a drug release supports in the form of sponges based on collagen, hyaluronic acid as a support and metronidazole as an antibiotic for the treatment of periodontitis. The sponges were characterized by FT-IR spectroscopy, water uptake, contact angle, SEM microscopy, in vitro metronidazole release analysis from sponges and data modeling.
The results showed that all the sponges had a porous structure with interconnected pores, the pore sizes being influenced by hyaluronic acid and metronidazole; the spongious structure became much more dense for samples with metronidazole content. All metronidazole-loaded sponges showed good surface wettability and an adequate swelling capacity for a suitable antimicrobial release at the periodontal pocket. The porous structures allow a controlled release, fast in the first hour, essential to control the initial microbial load at the periodontal level, which continues slowly in the following hours to ensure an effective treatment of periodontitis.
Correlating all physical-chemical and bio-pharmaceutical results obtained, a promising solution for periodontitis treatment could be a met-ronidazole-collagen-hyaluronic system consisting of 1% collagen, 1.5% metronidazole and 0.8% hyaluronic acid, and in vitro and in vivo tests are recommended to continue studies.
众所周知，牙周炎会影响牙龈及周围的结缔组织。牙龈组织中细菌引发的慢性炎症反应会导致牙齿与骨骼之间的胶原蛋白连接丧失，最终导致骨质流失。
在此背景下，本研究的目的是制备并表征一种以胶原蛋白为基础、透明质酸为载体、甲硝唑为抗生素的海绵状药物释放载体，用于治疗牙周炎。通过傅里叶变换红外光谱（FT-IR）、吸水率、接触角、扫描电子显微镜（SEM）、海绵体中甲硝唑的体外释放分析及数据建模对海绵体进行表征。
结果表明，所有海绵体均具有相互连通的多孔结构，孔径受透明质酸和甲硝唑影响；含甲硝唑的样品海绵状结构变得更加致密。所有负载甲硝唑的海绵体均表现出良好的表面润湿性和足够的溶胀能力，以便在牙周袋中实现合适的抗菌药物释放。多孔结构允许药物控释，在最初一小时内释放较快，这对于控制牙周部位的初始微生物负荷至关重要，随后数小时内缓慢释放以确保有效治疗牙周炎。
综合所获得的所有物理化学和生物药剂学结果，一种有前景的牙周炎治疗方案可能是由1%胶原蛋白、1.5%甲硝唑和0.8%透明质酸组成的甲硝唑-胶原蛋白-透明质酸体系，建议继续进行体外和体内试验以开展进一步研究。
