Zhong Bao, Zhao Yujuan, Gao Lei, Yang Ge, Gao Yansong, Li Fenglin, Li Shengyu
Institute of Agro-Food Technology, Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun 130033, China.
College of Food Science and Nutritional Engineering, Jilin Agriculture Science and Technology University, Jilin 132101, China.
Life (Basel). 2024 Oct 19;14(10):1334. doi: 10.3390/life14101334.
has been shown to have anticancer properties. However, there is limited research on the effects of postbiotic on colorectal cancer cell proliferation. Additionally, the exact mechanisms through which it influences apoptosis- and autophagy-related signaling pathways are yet to be thoroughly elucidated. This study explored the role of MZY531 as a postbiotic in inhibiting tumor growth by modulating apoptosis and autophagy in tumor cells. During the experimental period in the model group, tumors proliferated, tumor markers increased significantly, and immunofluorescence results showed that caspase-3 and terminal deoxynucleotidyl transferase dUTP nick-end labeling were significantly decreased. Conversely, supplementation with MZY531 postbiotics significantly reduced the levels of tumor markers carcinoembryonic antigen, colon cancer antigen, and extracellular protein kinase A and promoted cell apoptosis by increasing the caspase-3-positive count and terminal deoxynucleotidyl transferase dUTP nick-end labeling-positive cells in tumor tissue. Mechanistically, MZY531 postbiotics inhibit tumor growth through the modulation of the Bax/Bcl-2/caspase-3 and JAK2/STAT3 apoptosis pathways and PI3K/AKT/mTOR and TGF-β/SMAD4 cell autophagy pathways. MZY531 postbiotics had a more significant effect than that of MZY531 alone. Probiotics are expected to become effective natural functional foods for the treatment of colorectal cancer.
已被证明具有抗癌特性。然而，关于后生元对结肠癌细胞增殖影响的研究有限。此外，其影响凋亡和自噬相关信号通路的确切机制尚未得到充分阐明。本研究探讨了MZY531作为后生元通过调节肿瘤细胞凋亡和自噬来抑制肿瘤生长的作用。在模型组的实验期间，肿瘤增殖，肿瘤标志物显著增加，免疫荧光结果显示半胱天冬酶 - 3和末端脱氧核苷酸转移酶dUTP缺口末端标记显著减少。相反，补充MZY531后生元显著降低了肿瘤标志物癌胚抗原、结肠癌抗原和细胞外蛋白激酶A的水平，并通过增加肿瘤组织中半胱天冬酶 - 3阳性计数和末端脱氧核苷酸转移酶dUTP缺口末端标记阳性细胞来促进细胞凋亡。从机制上讲，MZY531后生元通过调节Bax/Bcl - 2/半胱天冬酶 - 3和JAK2/STAT3凋亡途径以及PI3K/AKT/mTOR和TGF - β/SMAD4细胞自噬途径来抑制肿瘤生长。MZY531后生元的效果比单独的MZY531更显著。益生菌有望成为治疗结肠癌的有效天然功能性食品。
