MB11 发酵对人参皂苷的转化：低含量人参皂苷的转化及增强抗结直肠癌细胞活性。
Transformation of Ginsenosides by MB11 Fermentation: Minor Ginsenosides Conversion and Enhancement of Anti-Colorectal Cancer Activity.
机构信息
Institute of Agricultural Products Processing Technology, Jilin Academy of Agricultural Sciences (Northeast Agriculture Research Center of China), Changchun 130033, China.
School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun 130117, China.
出版信息
Molecules. 2023 Dec 20;29(1):27. doi: 10.3390/molecules29010027.
The present study aimed to increase the content of minor ginsenosides and enhance the anti-colorectal cancer activity of ginsenosides via biotransformation by MB11 screened from fermented foods. A subcutaneous transplantation tumor model of murine colorectal cancer CT26 cells was established in mice to study the anticarcinogenic activities and mechanism of fermented total ginsenosides (FTGs). The results showed that MB11 fermentation increased the content of minor ginsenosides and decreased that of major ginsenosides. FTGs reduced the tumor weight and size compared with the model group. Immunofluorescence and TdT-mediated dUTP nick end labeling (TUNEL) analysis showed that FTGs significantly increase the number of caspase-3 cells in tumor tissue and induce cell apoptosis. Mechanically, FTGs activate AMPK/mTOR autophagy pathway and regulate JAK2/STAT3 and Bax/Bcl-2/caspase-3 apoptosis pathway. Overall, fermentation with MB11 enhanced minor ginsenosides in total ginsenosides, and FTGs induced subcutaneous transplantation tumor autophagy and apoptosis in mice.
本研究旨在通过从发酵食品中筛选出的 MB11 进行生物转化，增加人参中的低含量人参皂苷含量，并提高人参皂苷的抗结直肠癌活性。通过建立 CT26 细胞结直肠癌细胞皮下移植瘤模型，研究发酵总人参皂苷（FTGs）的抗癌活性及其机制。结果表明，MB11 发酵增加了低含量人参皂苷的含量，降低了高含量人参皂苷的含量。FTGs 与模型组相比，降低了肿瘤的重量和大小。免疫荧光和 TdT 介导的 dUTP 缺口末端标记（TUNEL）分析表明，FTGs 显著增加了肿瘤组织中 caspase-3 细胞的数量，并诱导细胞凋亡。从机制上看，FTGs 激活了 AMPK/mTOR 自噬通路，并调节了 JAK2/STAT3 和 Bax/Bcl-2/caspase-3 凋亡通路。总的来说，MB11 发酵增强了总人参皂苷中的低含量人参皂苷，FTGs 诱导了小鼠皮下移植瘤的自噬和凋亡。
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