Milović Tiana, Bulatović Vesna, Pezo Lato, Dramićanin Miroslav, Tomić Ana, Pezo Milada, Šovljanski Olja
Faculty of Technical Science, University of Novi Sad, Trg Dositeja Obradovića 6, 21000 Novi Sad, Serbia.
Institute of General and Physical Chemistry, University of Belgrade, Studentski Trg 12-16, 11000 Belgrade, Serbia.
Materials (Basel). 2024 Oct 11;17(20):4975. doi: 10.3390/ma17204975.
Using a Taguchi experimental design, this research focuses on utilizing indigenous bacteria from the Danube River to enhance the self-healing capabilities and structural integrity of cementitious materials. and were used as individual bacterium or in co-culture, with a concentration of 8 logs CFU, while the humidity variation involved testing wet and wet-dry conditions. Additionally, artificial neural network (ANN) modeling of the compressive strength of cement samples results in improvements in compressive strength, particularly under wet-dry conditions. By inducing targeted bacterial activity, the formation of calcium carbonate precipitates was initiated, which effectively sealed formed cracks, thus restoring and even enhancing the material's strength. In addition to short-term improvements, this study also evaluates long-term improvements, with compressive strength measured over periods extending to 180 days. The results demonstrate sustained self-healing capabilities and strength improvements under varied environmental conditions, emphasizing the potential for long-term application in real-world infrastructure. This study also explores the role of environmental conditions, such as wet and wet-dry cycles, in optimizing the self-healing process, revealing that cyclic exposure conditions further improve the efficiency of strength recovery. The findings suggest that autochthonous bacterial co-cultures can be a viable solution for enhancing the durability and lifespan of concrete structures. This research provides a foundation for further exploration into bio-based self-healing mechanisms and their practical applications in the concrete industry.
本研究采用田口实验设计，重点关注利用多瑙河的本土细菌来增强胶凝材料的自愈能力和结构完整性。将[细菌名称1]和[细菌名称2]作为单一细菌或混合培养使用，浓度为8 log CFU，同时湿度变化涉及测试潮湿和干湿条件。此外，对水泥样品抗压强度的人工神经网络（ANN）建模导致抗压强度有所提高，特别是在干湿条件下。通过诱导有针对性的细菌活动，引发了碳酸钙沉淀的形成，有效地密封了形成的裂缝，从而恢复甚至提高了材料的强度。除了短期改善外，本研究还评估了长期改善情况，抗压强度的测量时间延长至180天。结果表明，在不同环境条件下具有持续的自愈能力和强度提高，强调了在实际基础设施中长期应用的潜力。本研究还探讨了潮湿和干湿循环等环境条件在优化自愈过程中的作用，揭示了循环暴露条件进一步提高了强度恢复效率。研究结果表明本土细菌混合培养可以成为提高混凝土结构耐久性和寿命的可行解决方案。本研究为进一步探索基于生物的自愈机制及其在混凝土行业的实际应用奠定了基础。
