Sichuan Engineering Research Center for Crop Strip Intercropping System and College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China.
Viruses. 2024 Oct 11;16(10):1600. doi: 10.3390/v16101600.
Actinidia chlorotic ringspot-associated virus (AcCRaV, ) is prevalent in Chinese kiwifruit, leading to substantial yield reduction. The intricate nature of symptoms presents diagnostic challenges, underscoring the necessity for a rapid and accurate detection method that facilitates effective control. In this investigation, AcCRaV isolates from key kiwi-producing regions in Sichuan province were collected and analyzed, with representative strains chosen as experimental materials. Primers targeting the nucleoprotein gene of AcCRaV were designed, and their codon usage was optimized to enhance performance. Various serological methods utilizing polyclonal antibodies were developed, including ELISA, dot immunobinding assay, and AcCRaV-specific gold immunochromatographic bands (AcCRaV-GICS). Field samples exhibited high specificity and sensitivity when tested using these methods. Furthermore, the results obtained from a large number of field samples are consistent with those derived from RT-PCR analysis, further validating the applicability of our approach. A detection method capable of handling a large volume of field samples infected with AcCRaV is currently lacking; thus, our system construction provides an important reference for addressing this gap.
中华猕猴桃细菌性斑点病相关病毒（AcCRaV）在中国猕猴桃中普遍存在，导致产量大幅下降。症状的复杂性给诊断带来了挑战，这突显了快速准确的检测方法的必要性，以促进有效的控制。在本研究中，收集和分析了来自四川省主要猕猴桃产区的 AcCRaV 分离株，选择代表性菌株作为实验材料。设计了针对 AcCRaV 核蛋白基因的引物，并对其密码子使用进行了优化以提高性能。利用多克隆抗体开发了各种血清学方法，包括 ELISA、斑点免疫结合试验和 AcCRaV 特异性金免疫层析带（AcCRaV-GICS）。这些方法对田间样本的检测具有较高的特异性和灵敏度。此外，大量田间样本的检测结果与 RT-PCR 分析结果一致，进一步验证了该方法的适用性。目前缺乏一种能够处理大量感染 AcCRaV 的田间样本的检测方法，因此，我们的系统构建为解决这一空白提供了重要参考。
