Kurskiĭ M D, Tugaĭ V A, Usatiuk P V
Ukr Biokhim Zh (1978). 1986 Jan-Feb;58(1):56-62.
Studies with the use of [3H]acetate as an delta pH-indicator have established that pH in the native vesicles of sarcoplasmic reticulum is by 0.54 unit lower, than its extra-molecular value (6.5 units). The double [3H] and radioactive [3H] and [45Ca2+] labels were used to show that Ca2+ transport into the sarcoplasmic reticulum vesicles is accompanied by an increase in intravesicular pH. Carbonylcyanide-m-chlorophenylhydrazone, a protonophore, stimulates the equalization of the pH gradient (H+ removal) which is not accompanied by changes in the Ca2+ transport. In the presence of ionophore A23187 Ca2+ and [3H]acetate do not accumulate in vesicles in the ATP-dependent process. This indicates H+ removal from the vesicles only when there is the Ca2+ gradient creation and the absence of the close conjugation of Ca3+/2H+ realized by Ca2+-ATPase of sarcoplasmic reticulum.
使用[3H]乙酸盐作为δpH指示剂的研究表明，肌浆网天然囊泡中的pH比其分子外值（6.5个单位）低0.54个单位。使用双[3H]以及放射性[3H]和[45Ca2+]标记来表明，Ca2+转运到肌浆网囊泡中伴随着囊泡内pH的升高。羰基氰化物间氯苯腙，一种质子载体，刺激pH梯度的平衡（H+去除），这并不伴随着Ca2+转运的变化。在离子载体A23187存在的情况下，Ca2+和[3H]乙酸盐在依赖ATP的过程中不会在囊泡中积累。这表明只有当存在Ca2+梯度形成且肌浆网Ca2+-ATP酶实现的Ca3+/2H+紧密偶联不存在时，H+才会从囊泡中去除。
