Program of Veterinary Medicine, School of Veterinary Medicine and Biomedical Sciences, IPB University, Jalan Agathis, Kampus IPB Dramaga, Bogor, West Java, Indonesia.
Postgraduate Program of Biology Reproduction, School of Veterinary Medicine and Biomedical Sciences, IPB University, Jalan Agathis, Kampus IPB Dramaga, Bogor, West Java, Indonesia.
Reprod Domest Anim. 2024 Oct;59(10):e14736. doi: 10.1111/rda.14736.
This preliminary study evaluated the biocompatibility of a novel degradable intravaginal plug contraceptive composed of PEG 4000 and chitosan in cats using haematological profiling and vaginal cytology. Five healthy, non-pregnant female cats were fully anaesthetised and fitted with an intravaginal plug (10 × 0.3 mm) using an applicator, following oestrogen administration 3 h prior. Blood samples were collected from the cephalic vein on days 0 (pre-insertion) and 3 and 7 (post-insertion). Vaginal cytology examinations were conducted on day 0 (pre- and post-oestrogen injection) and days 1, 3 and 7 post-insertion. Haematological parameters, including red blood cell count, haemoglobin levels, haematocrit values, total white blood cell count and differentiation, showed no significant changes after contraceptive insertion (p > 0.05). Vaginal cytology indicated an acute inflammatory response in one out of five subjects on day three post-insertion. The distribution of vaginal epithelial cells (parabasal, intermediate and superficial) remained unaffected by contraception. Oestrogen injection resulted in the dominance of superficial cells up to day 7 of observation (p < 0.05). Overall, PEG 4000 and chitosan-based intravaginal plug contraceptives demonstrated sufficient biocompatibility, indicating their potential as viable contraceptive options for feline use.
本初步研究评估了一种由聚乙二醇 4000 和壳聚糖组成的新型可降解阴道避孕栓在猫体内的生物相容性，方法是通过血液学分析和阴道细胞学检查。在雌激素给药 3 小时后，将 5 只健康、未怀孕的雌性猫完全麻醉，并使用阴道给药器插入阴道避孕栓（10×0.3mm）。在插入前（第 0 天）和插入后第 3 天和第 7 天，从头静脉采集血液样本。在插入前（雌激素注射前后）和插入后第 1、3 和 7 天进行阴道细胞学检查。红细胞计数、血红蛋白水平、红细胞压积值、总白细胞计数和分类等血液学参数在避孕栓插入后没有明显变化（p>0.05）。阴道细胞学检查显示，在插入后第 3 天，有 1 只猫出现急性炎症反应。阴道上皮细胞（基底细胞、中间细胞和表层细胞）的分布不受避孕措施的影响。雌激素注射导致表层细胞的优势在观察的第 7 天仍持续（p<0.05）。总的来说，基于聚乙二醇 4000 和壳聚糖的阴道避孕栓表现出足够的生物相容性，这表明它们有潜力成为猫用可行的避孕选择。
