Zhang Gan, Liu Tingting, Chen Jiawei, Yu Sha, Zheng Zulan, Deng Shengliang, Peng Juan, Lai Weihua
State Key Laboratory of Food Science and Resources, Nanchang University, 330047, Nanchang, China.
Institute of Microbiology, Jiangxi Academy of Sciences, 330096, Nanchang, China.
Talanta. 2025 Feb 1;283:127092. doi: 10.1016/j.talanta.2024.127092. Epub 2024 Oct 22.
T-2 toxin, the strongest toxic of type A trichothecenes, widely exists in barley, wheat, corn, oats, and even human food, which is urgent to establish a rapid and sensitive detection method for T-2 toxin. Herein, we developed a fluorescent lateral flow immunoassay (LFIA) for sensitive detection of T-2 toxin. Specially, the monoclonal antibody (mAb) was prepared by immunizing mice and cell fusion, and exhibited a low half maximal inhibitory concentration (IC) of 0.482 ng mL. In addition, an aggregation-induced emission (AIE)-based metal-organic framework (HfMOFs) with excellent fluorescence performance (QY: 72.5 %) was developed as reporter. Benefiting from these advantages, the limit of detection (LoD) of HfMOFs-LFIA was 0.0487 ng mL, which was 7.7-fold lower than traditional nanoparticles-based LFIA (0.375 ng mL). In addition, HfMOFs-LFIA was used for the detection of T-2 toxin in actual samples and showed satisfactory recoveries (71.3 %-123.7 %) with coefficient of variation (2.96 %-14.07 %). Overall, the dual-improvement in mAb and label might power the development in LFIA, achieving innovative prospects for LFIA in food safety fields.
T-2毒素是A型单端孢霉烯族化合物中毒性最强的一种，广泛存在于大麦、小麦、玉米、燕麦甚至人类食品中，因此迫切需要建立一种快速灵敏的T-2毒素检测方法。在此，我们开发了一种用于灵敏检测T-2毒素的荧光侧向流动免疫分析（LFIA）方法。具体而言，通过免疫小鼠和细胞融合制备了单克隆抗体（mAb），其半数最大抑制浓度（IC）低至0.482 ng/mL。此外，还开发了一种具有优异荧光性能（量子产率：72.5%）的基于聚集诱导发光（AIE）的金属有机框架（HfMOFs）作为报告分子。受益于这些优势，HfMOFs-LFIA的检测限（LoD）为0.0487 ng/mL，比传统的基于纳米颗粒的LFIA（0.375 ng/mL）低7.7倍。此外，HfMOFs-LFIA用于实际样品中T-2毒素的检测，回收率令人满意（71.3%-123.7%），变异系数为（2.96%-14.07%）。总体而言，单克隆抗体和标记物的双重改进可能推动LFIA的发展，为LFIA在食品安全领域带来创新前景。
