Li Yang, Yan Xinyue, Qin Tianqi, Gan Ying, Li Na, Zheng Chunshan
State Key Laboratory of Mining Response and Disaster Prevention and Control in Deep Coal Mines, Anhui University of Science and Technology, Huainan, 232001, China.
State Key Laboratory of Mining Response and Disaster Prevention and Control in Deep Coal Mines, Anhui University of Science and Technology, Huainan, 232001, China.
J Environ Manage. 2024 Nov;370:123063. doi: 10.1016/j.jenvman.2024.123063. Epub 2024 Oct 28.
The blue algae can be used as a nitrogen agent for promoting biological coalbed methane, but its applicability and microbial mechanism in different microbial enhanced coalbed methane technologies kept unknown. This study evaluated the methanogenic efficiency of blue algae addition with a mass ratio of 10% under fermentative degradation and microbial electrolytic cell technologies, and studied the changes of coal microstructure, surface functional groups, organic components and microbiome. The results showed that the algae addition affected the micro-concave-convex structure, non-uniform distribution of micro-particles and micro-cracks of coals, and finally increased the methanogenic rate by 1.74-2.66 times. The algae addition mainly affected the coal organic components including hydroxyl structure, hydrocarbon structure, aliphatic oxygen-containing functional groups and aromatic structure, as well increased the humus acids and microbial metabolites in fermentation broth; among them, the increased metabolites showed great differences between different technologies. The algae addition mainly increased the genera belonging to phylum Bacillota (such as Bacillus and Clostridium) and methanogens (Methanosarcina and Methanoculleus). These Bacillota groups could degrade organic matter into acetate and methanol via pathways of glycolysis and benzoate degradation, which provided substrates for such methanogens. This study strengthened the effectiveness of blue algae in enhancing technologies for biological coalbed methane.
蓝藻可作为促进生物煤层气生成的氮源，但在不同的微生物强化煤层气技术中其适用性和微生物作用机制尚不清楚。本研究评估了在发酵降解和微生物电解池技术中添加质量比为10%的蓝藻时的产甲烷效率，并研究了煤微观结构、表面官能团、有机成分和微生物群落的变化。结果表明，添加蓝藻影响了煤的微凹凸结构、微粒分布不均和微裂缝，最终使产甲烷率提高了1.74 - 2.66倍。添加蓝藻主要影响了煤的有机成分，包括羟基结构、烃类结构、脂肪族含氧官能团和芳香结构，同时也增加了发酵液中的腐殖酸和微生物代谢产物；其中，不同技术中增加的代谢产物差异很大。添加蓝藻主要增加了厚壁菌门（如芽孢杆菌属和梭菌属）和产甲烷菌（甲烷八叠球菌属和甲烷小囊菌属）的属。这些厚壁菌门菌群可通过糖酵解和苯甲酸降解途径将有机物降解为乙酸和甲醇，为这类产甲烷菌提供底物。本研究强化了蓝藻在生物煤层气强化技术中的有效性。
