Roe McKenna D, Hood Grace, Sterling Spencer L, Yan Lianying, Boré Joseph Akoi, Tipton Tom, Thompson Craig, Carroll Miles W, Laing Eric D
Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA.
Centre for Human Genetics & Pandemic Sciences Institute, University of Oxford, Oxford, UK.
J Virol Methods. 2025 Jan;331:115057. doi: 10.1016/j.jviromet.2024.115057. Epub 2024 Oct 24.
Serological surveillance in animal and human hosts can be a cost-effective strategy for orthoebolavirus detection, but is challenged by accurate estimates of seroprevalence, potential pauci-symptomatic disease presentation, and antigenic cross-reactivity. Here, we describe the use of an envelope glycoprotein (GP)-based multiplex microsphere immunoassay, consisting of nine filovirus GP antigens for the detection of anti-Ebola virus (EBOV) antibodies in a well-characterized cohort of Guinean Ebola virus disease (EVD) survivors and contacts from the 2013 - 2016 West African EVD outbreak. We examined sensitivity and specificity for the detection of anti-EBOV antibodies by GP expressed as recombinant trimeric ectodomains, yielding an assay performance of 95.96 % sensitivity and 98.61 % specificity. Additionally, agreement between the multiplex test and a whole virus ELISA and virus neutralization test showed strong correlations. The results demonstrate that this filovirus multiplex test is a sensitive tool for high-throughput serosurveillance.
在动物和人类宿主中进行血清学监测可能是检测正ebolavirus的一种具有成本效益的策略，但它受到血清阳性率的准确估计、潜在的轻症疾病表现以及抗原交叉反应性的挑战。在这里，我们描述了一种基于包膜糖蛋白（GP）的多重微球免疫测定法的应用，该方法由九种丝状病毒GP抗原组成，用于在一组特征明确的几内亚埃博拉病毒病（EVD）幸存者以及2013 - 2016年西非EVD疫情中的接触者中检测抗埃博拉病毒（EBOV）抗体。我们通过表达为重组三聚体外结构域的GP检测抗EBOV抗体的敏感性和特异性，该检测方法的检测性能为敏感性95.96%，特异性98.61%。此外，多重检测与全病毒ELISA和病毒中和试验之间的一致性显示出很强的相关性。结果表明，这种丝状病毒多重检测是一种用于高通量血清学监测的灵敏工具。
