Zhang Qianqian, Feng Yifan, Zhao Jixiang, Sun Shuhui, Zheng Tingting, Wang Jinrui, Chen Huan, Ye Hanyi, Lv Shun, Zhang Yinghua, Wang Siming, Li Ying, Dong Zhengqi
State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, PR China; College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China.
State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, PR China.
Int J Biol Macromol. 2024 Dec;282(Pt 2):136877. doi: 10.1016/j.ijbiomac.2024.136877. Epub 2024 Oct 24.
The emergence of drug-resistant bacteria poses significant challenges in wound treatment. Antimicrobial photodynamic therapy has emerged as an effective approach to eliminating bacteria by inducing oxidative stress without causing drug resistance. Here, we developed a natural hyaluronic acid (HA)-gallic acid (GA) conjugation-based hydrogel combined with herbal photosensitizer-caffeic acid (CA), which exhibits self-healing ability, shape adaptability, biodegradability, and robust tissue adhesion. Under exposure to 400 nm light, caffeic acid acts as a photosensitizer, generating reactive oxygen species and oxidative damage to bacterial cell membranes. Furthermore, the presence of GA and CA displayed a continuous inhibitory effect on bacterial growth, along with antioxidant properties that promote wound healing even after the cessation of light exposure. The antibacterial mechanism of the HA-GA/CA hydrogel against MRSA, S. aureus, and E. coli was investigated through various assays measuring ATP levels, Zeta potential, hydroxyl radicals (·OH) generated by light irradiation, and biofilm clearance rate. Additionally, hydrogel's application in treating MRSA-infected wounds in mice under light irradiation demonstrated rapid wound-healing effects and biocompatibility. Overall, HA-GA/CA hydrogel provides a sustainable, antibiotic-free alternative for treating MRSA-infected wounds.
耐药细菌的出现给伤口治疗带来了重大挑战。抗菌光动力疗法已成为一种有效的除菌方法，它通过诱导氧化应激来消除细菌，而不会产生耐药性。在此，我们开发了一种基于天然透明质酸（HA）-没食子酸（GA）共轭的水凝胶，并结合了草药光敏剂咖啡酸（CA），该水凝胶具有自愈能力、形状适应性、生物可降解性和强大的组织粘附性。在400纳米光照射下，咖啡酸作为光敏剂，产生活性氧并对细菌细胞膜造成氧化损伤。此外，GA和CA的存在对细菌生长具有持续抑制作用，并且具有抗氧化特性，即使在光照停止后也能促进伤口愈合。通过各种测定ATP水平、zeta电位、光照射产生的羟基自由基（·OH）以及生物膜清除率的试验，研究了HA-GA/CA水凝胶对耐甲氧西林金黄色葡萄球菌（MRSA）、金黄色葡萄球菌和大肠杆菌的抗菌机制。此外，水凝胶在光照下治疗小鼠MRSA感染伤口的应用显示出快速的伤口愈合效果和生物相容性。总体而言，HA-GA/CA水凝胶为治疗MRSA感染伤口提供了一种可持续的、无抗生素的替代方案。
