Li Lihua, Ren Li, Zhao Qiuhan, Xu Ke, Wu Qiushuang, Su Qianru, Li Xin, Lü Xin, Wang Li
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China; Shaanxi Engineering Research Centre of Dairy Products Quality, Safety and Health, Shaanxi, China.
Carbohydr Polym. 2025 Mar 15;352:123179. doi: 10.1016/j.carbpol.2024.123179. Epub 2024 Dec 22.
The management of wounds infected with drug-resistant bacteria represents a significant challenge to public health globally. Nanotechnology-functionalized photothermal hydrogel with good thermal stability, biocompatibility and tissue adhesion exhibits great potential in treating these infected wounds. Herein, a novel photothermal hydrogel (mCS-Cu-Ser) was prepared through in situ mineralization in the hydrogel networks and ion cross-linking driven by copper ions (∼3 mM). Self-assembling polyphosphate sericin nanoparticles (Ser NPs) formed by an ultrasound-assisted anti-solvent method were as mineralization templates and gallic acid-grafted chitosan (mCS) was prepared as the sole matrix. Grafting of polyphenols and cross-linking of copper ions endowed mCS-Cu-Ser with injectable, skin-adhesive and self-healing characteristics. Due to the nonradiative relaxation of Cu electron-hole pairs of copper phosphate on the surface of Ser NPs and the molecular thermo-vibrational effect of the mCS-Cu complex, mCS-Cu-Ser rapidly warmed up to 50 °C within one minute under near-infrared (NIR) irradiation. Integrating such excellent photothermal properties with antimicrobial activity and intracellular reactive oxygen species scavenging from mCS, mCS-Cu-Ser + NIR effectively accelerated methicillin-resistant Staphylococcus aureus (MRSA) infected wound healing. This work develops a novel dressing for the treatment of MRSA-infected wounds and provides some reference for the preparation of multifunctional acid-free chitosan hydrogels.
耐药菌感染伤口的处理是全球公共卫生面临的重大挑战。具有良好热稳定性、生物相容性和组织粘附性的纳米技术功能化光热水凝胶在治疗这些感染伤口方面具有巨大潜力。在此，通过水凝胶网络中的原位矿化和铜离子（约3 mM）驱动的离子交联制备了一种新型光热水凝胶（mCS-Cu-Ser）。通过超声辅助反溶剂法形成的自组装聚磷酸丝胶纳米颗粒（Ser NPs）作为矿化模板，制备了没食子酸接枝壳聚糖（mCS）作为唯一基质。多酚的接枝和铜离子的交联赋予mCS-Cu-Ser可注射、皮肤粘附和自愈特性。由于Ser NPs表面磷酸铜的铜电子-空穴对的非辐射弛豫以及mCS-Cu络合物的分子热振动效应，mCS-Cu-Ser在近红外（NIR）照射下一分钟内迅速升温至50°C。将这种优异的光热性能与抗菌活性以及mCS清除细胞内活性氧的能力相结合，mCS-Cu-Ser + NIR有效加速了耐甲氧西林金黄色葡萄球菌（MRSA）感染伤口的愈合。这项工作开发了一种用于治疗MRSA感染伤口的新型敷料，并为多功能无酸壳聚糖水凝胶的制备提供了一些参考。
