Hounsou Konei Emangbondji, Sonibare Mubo Adeola, Elufioye Taiwo Olayemi
Medicinal Plants Research and Drug Development Programme, Pan African University Life and Earth Sciences Institute (including Health and Agriculture), PAULESI, University of Ibadan, 200005 Ibadan, Nigeria.
Medicinal Plants Research and Drug Development Programme, Pan African University Life and Earth Sciences Institute (including Health and Agriculture), PAULESI, University of Ibadan, 200005 Ibadan, Nigeria; Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, 200005 Ibadan, Nigeria.
Ann Pharm Fr. 2025 Mar;83(2):331-341. doi: 10.1016/j.pharma.2024.10.007. Epub 2024 Oct 24.
Recent advancements in scientific understanding of free radicals have stimulated progress in medicine. Antioxidants are known to neutralize free radicals by giving up electrons. The current research was carried out to explore the antioxidant capabilities and phytochemical composition of a synergy-based combined extracts of Spondias mombin,Spilanthes filicaulis, and Piper guineense, with the goal to determine the optimal ratio for the most effective antioxidant activity, suitable for herbal product development.
Combined extracts H1 and H2 were obtained through aqueous maceration of S. mombin leaves, S. filicaulis plants, and P. guineense fruits. Antioxidant activity of combined extract was evaluated in 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and ferric ion reducing power (FRAP) assay. Evaluations included total phenolic and flavonoid content determination. Furthermore, the phytochemical constituents were determined using Gas Chromatography-Mass Spectrometry (GC-MS).
The combined extract H1 exhibited better free radical scavenging ability in DPPH (IC=72.87±0.63μg/mL) than H2 (IC=150.2±1.08μg/mL). The FRAP assay results revealed its highest reducing power (12.385±0.002 AAE/mg/g) compared to H2 (11.657±0.002 AAE/mg/g). Additionally, H1 had higher of both total phenolic and flavonoid content. GC-MS profiling revealed 28 compounds within H1 and 17 compounds within H2.
This study demonstrated that the combined extract H1 has significant antioxidant potential, with consistently positive results across all conducted assays. It contains a variety of phytochemical constituents, mainly phenolic and flavonoids that could be explored in pharmaceutical industries to develop antioxidant agents.
近年来，对自由基的科学认识取得了进展，推动了医学的进步。已知抗氧化剂通过给出电子来中和自由基。本研究旨在探索基于协同作用的腰果树、丝状金纽扣和几内亚胡椒组合提取物的抗氧化能力和植物化学成分，以确定具有最有效抗氧化活性的最佳比例，适用于草药产品开发。
通过对腰果树叶片、丝状金纽扣植株和几内亚胡椒果实进行水浸提取，得到组合提取物H1和H2。采用2,2-二苯基-1-苦基肼（DPPH）法和铁离子还原能力（FRAP）法评估组合提取物的抗氧化活性。评估内容包括总酚和黄酮含量的测定。此外，使用气相色谱-质谱联用仪（GC-MS）测定植物化学成分。
组合提取物H1在DPPH法中表现出比H2更好的自由基清除能力（IC=72.87±0.63μg/mL，而H2为IC=150.2±1.08μg/mL）。FRAP法测定结果显示，与H2（11.657±0.002 AAE/mg/g）相比，H1具有最高的还原能力（12.385±0.002 AAE/mg/g）。此外，H1的总酚和黄酮含量均更高。GC-MS分析表明，H1中有28种化合物，H2中有17种化合物。
本研究表明，组合提取物H1具有显著的抗氧化潜力，在所有进行的测定中均获得一致的阳性结果。它含有多种植物化学成分，主要是酚类和黄酮类，可在制药行业中用于开发抗氧化剂。
