Pinheiro Maria R, Carvalho Maria I, Oliveira Luís M
Institute for Systems and Computer Engineering, Technology and Science (INESC TEC), Porto, Portugal.
Department of Electrical and Computer Engineering, Faculty of Engineering, Porto University, Porto, Portugal.
J Biophotonics. 2024 Oct 26:e202400257. doi: 10.1002/jbio.202400257.
Computer simulations, which are performed at a single wavelength at a time, have been traditionally used to estimate the optical properties of tissues. The results of these simulations need to be interpolated. For a broadband estimation of tissue optical properties, the use of computer simulations becomes time consuming and computer demanding. When spectral measurements are available for a tissue, the use of the photon diffusion approximation can be done to perform simple and direct calculations to obtain the broadband spectra of some optical properties. The additional estimation of the reduced scattering coefficient at a small number of discrete wavelengths allows to perform further calculations to obtain the spectra of other optical properties. This study used spectral measurements from the heart muscle to explain the calculation pipeline to obtain a complete set of the spectral optical properties and to show its versatility for use with other tissues for various biophotonics applications.
传统上，计算机模拟是一次在单一波长下进行的，用于估计组织的光学特性。这些模拟结果需要进行插值。对于组织光学特性的宽带估计，使用计算机模拟变得既耗时又对计算机要求很高。当有组织的光谱测量数据时，可以使用光子扩散近似来进行简单直接的计算，以获得一些光学特性的宽带光谱。在少数离散波长处对约化散射系数进行额外估计，可以进行进一步计算以获得其他光学特性的光谱。本研究使用来自心肌的光谱测量数据来解释计算流程，以获得完整的光谱光学特性集，并展示其在各种生物光子学应用中与其他组织一起使用的通用性。
