Cancer Treatment Center, Affiliated Hospital of Beihua University, Jilin 132011, China.
Special Service Personnel Health Management Department, Chinese People's Liberation Army Strategic Support Force Special Medical Center, Beijing 100101, China.
J Environ Pathol Toxicol Oncol. 2025;44(1):1-9. doi: 10.1615/JEnvironPatholToxicolOncol.2024053299.
The incidence of breast cancer, a malignant tumor that causes more harm to women, is increasing year by year, affecting women at a younger age. This paper describes the possible practical significance of lncRNA EBLN3P (EBLN3P) in predicting the prognosis of invasive breast cancer. EBLN3P and miR-144-3p levels in tissues and cells were detected by real-time quantitative PCR (RT-qPCR). The association between EBLN3P expression and prognosis of invasive breast cancer was investigated using Cox multivariate regression and Kaplan-Meier curve. The growth efficacy of EBLN3P expression on invasive breast cancer cells was evaluated by Cell Counting kit-8 (CCK-8) method and Transwell method, and the mechanism of EBLN3P targeting miR-144-3p was further studied. EBLN3P was elevated in invasive breast cancer, whereas survival rates were lower in patients with high EBLN3P level. EBLN3P directly targeted miR-144-3p to participate in the mechanism of invasive breast cancer, and EBLN3P knockdown had an inhibitory effect on tumor cells. Silencing EBLN3P inhibited the advancement of invasive breast cancer and was expected to be a promising therapeutic target for clinical intervention and prognosis.
乳腺癌的发病率逐年上升，这种恶性肿瘤对女性的危害更大，且发病年龄日趋年轻化。本文描述了 lncRNA EBLN3P（EBLN3P）在预测浸润性乳腺癌预后方面的可能实际意义。通过实时定量 PCR（RT-qPCR）检测组织和细胞中的 EBLN3P 和 miR-144-3p 水平。使用 Cox 多变量回归和 Kaplan-Meier 曲线研究 EBLN3P 表达与浸润性乳腺癌预后的相关性。通过细胞计数试剂盒-8（CCK-8）法和 Transwell 法评估 EBLN3P 表达对浸润性乳腺癌细胞生长效果的影响，并进一步研究 EBLN3P 靶向 miR-144-3p 的机制。EBLN3P 在浸润性乳腺癌中升高，而 EBLN3P 水平高的患者生存率较低。EBLN3P 通过直接靶向 miR-144-3p 参与浸润性乳腺癌的发生机制，EBLN3P 敲低对肿瘤细胞具有抑制作用。沉默 EBLN3P 抑制了浸润性乳腺癌的进展，有望成为临床干预和预后的有前途的治疗靶点。
