Department of Pulmonary and Critical Care Medicine, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, 210008, China.
Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University.
J Environ Pathol Toxicol Oncol. 2025;44(1):11-20. doi: 10.1615/JEnvironPatholToxicolOncol.2024053394.
As one of the three major malignant tumors, lung adenocarcinoma (LUAD), with its rapid progression and high mortality rate, has become the most dangerous factor endangering human health. This study aims to explore new potential molecular targets, explore the regulatory role of lncRNA APTR in LUAD, and provide a more theoretical basis for the selection of LUAD therapeutic targets. The expression of APTR in LUAD was detected by PCR experiments, and the relationship between APTR and patients' clinical conditions and prognosis was analyzed by chi-square test, multifactor Cox regression, and Kaplan-Meier. The interaction between APTR and miR-298 and the regulation of LUAD cellular activities by APTR/miR-298 were explored by the luciferase reporter gene system. APTR expression was found to be upregulated in LUAD tissues and cells, and the expression of APTR was revealed to be substantially linked with lymph node metastases and TNM stage. High expression of LUAD also predicted a poor prognosis for patients. Downregulation of APTR expression significantly inhibited the activities of LUAD cells. In addition, APTR targeted miR-298 and negatively regulated miR-298 expression. The inhibitory effect of APTR knockdown on LUAD cell activity was also reversed after transfection with miR-298 inhibitor. Increasing expression of APTR is associated with patients' poor prognosis, APTR targets miR-298 and promotes LUAD cellular activity through negative regulation of miR-298.
作为三大恶性肿瘤之一，肺腺癌（LUAD）具有进展迅速、死亡率高的特点，已成为危害人类健康的最危险因素。本研究旨在探索新的潜在分子靶点，探讨 lncRNA APTR 在 LUAD 中的调控作用，为 LUAD 治疗靶点的选择提供更充分的理论依据。通过 PCR 实验检测 APTR 在 LUAD 中的表达，通过卡方检验、多因素 Cox 回归和 Kaplan-Meier 分析 APTR 与患者临床状况和预后的关系。通过荧光素酶报告基因系统探索 APTR 与 miR-298 的相互作用以及 APTR/miR-298 对 LUAD 细胞活性的调节。结果发现，APTR 在 LUAD 组织和细胞中表达上调，APTR 的表达与淋巴结转移和 TNM 分期密切相关。LUAD 高表达也预示着患者预后不良。APTR 表达下调显著抑制 LUAD 细胞的活性。此外，APTR 靶向 miR-298 并负调控 miR-298 的表达。转染 miR-298 抑制剂后，APTR 敲低对 LUAD 细胞活性的抑制作用也得到逆转。APTR 表达的增加与患者预后不良有关，APTR 通过负调控 miR-298 靶向 miR-298 并促进 LUAD 细胞活性。
