Fu Rui, Zhu Haijun, Ding Chong, Xu Guizhi
Key Laboratory of Bioelectromagnetic and Neural Engineering of Hebei Province, Hebei University of Technology, Tianjin 300130, P.R.China.
State Key Laboratory of Reliability and Intelligence of Electrical Equipment, Hebei University of Technology, Tianjin 300130, P.R.China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2024 Oct 25;41(5):935-944. doi: 10.7507/1001-5515.202312017.
Transcranial magnetic stimulation (TMS) as a non-invasive neuroregulatory technique has been applied in the clinical treatment of neurological and psychiatric diseases. However, the stimulation effects and neural regulatory mechanisms of TMS with different frequencies and modes are not yet clear. This article explores the effects of different frequency repetitive transcranial magnetic stimulation (rTMS) and burst transcranial magnetic stimulation (bTMS) on memory function and neuronal excitability in mice from the perspective of neuroelectrophysiology. In this experiment, 42 Kunming mice aged 8 weeks were randomly divided into pseudo stimulation group and stimulation groups. The stimulation group included rTMS stimulation groups with different frequencies (1, 5, 10 Hz), and bTMS stimulation groups with different frequencies (1, 5, 10 Hz). Among them, the stimulation group received continuous stimulation for 14 days. After the stimulation, the mice underwent new object recognition and platform jumping experiment to test their memory ability. Subsequently, brain slice patch clamp experiment was conducted to analyze the excitability of granulosa cells in the dentate gyrus (DG) of mice. The results showed that compared with the pseudo stimulation group, high-frequency (5, 10 Hz) rTMS and bTMS could improve the memory ability and neuronal excitability of mice, while low-frequency (1 Hz) rTMS and bTMS have no significant effect. For the two stimulation modes at the same frequency, their effects on memory function and neuronal excitability of mice have no significant difference. The results of this study suggest that high-frequency TMS can improve memory function in mice by increasing the excitability of hippocampal DG granule neurons. This article provides experimental and theoretical basis for the mechanism research and clinical application of TMS in improving cognitive function.
经颅磁刺激（TMS）作为一种非侵入性神经调节技术已应用于神经和精神疾病的临床治疗。然而，不同频率和模式的TMS的刺激效果和神经调节机制尚不清楚。本文从神经电生理学角度探讨不同频率重复经颅磁刺激（rTMS）和爆发式经颅磁刺激（bTMS）对小鼠记忆功能和神经元兴奋性的影响。在本实验中，将42只8周龄的昆明小鼠随机分为假刺激组和刺激组。刺激组包括不同频率（1、5、10Hz）的rTMS刺激组和不同频率（1、5、10Hz）的bTMS刺激组。其中，刺激组接受连续14天的刺激。刺激后，对小鼠进行新物体识别和平台跳跃实验以测试其记忆能力。随后，进行脑片膜片钳实验以分析小鼠齿状回（DG）颗粒细胞的兴奋性。结果表明，与假刺激组相比，高频（5、10Hz）rTMS和bTMS可提高小鼠的记忆能力和神经元兴奋性，而低频（1Hz）rTMS和bTMS无显著影响。对于相同频率的两种刺激模式，它们对小鼠记忆功能和神经元兴奋性的影响无显著差异。本研究结果表明，高频TMS可通过增加海马DG颗粒神经元的兴奋性来改善小鼠的记忆功能。本文为TMS改善认知功能的机制研究和临床应用提供了实验和理论依据。
