Akagi Misako, Nakamura Noriko, Tanaka Yoshikazu
Research Institute, Suntory Global Innovation Center Ltd.
Plant Biotechnol (Tokyo). 2024 Jun 25;41(2):147-151. doi: 10.5511/plantbiotechnology.24.0409a.
The members of glutathione -transferase (GST) belonging to the Phi class of the GST family are known to play a role in anthocyanin transport to the vacuole. We isolated a GST orthologue from the torenia petal cDNA library. Transgenic plants transcribing GST double stranded RNA were generated from a torenia cultivar having blue flowers. These plants exhibited a range of flower colors, from blue to almost white. Quantitative RT-PCR confirmed the downregulation of the GST transcript, accompanied by a decrease in anthocyanin levels in the petals of the transgenic plants, whereas flavone levels remained unchanged. These results suggest that GST is involved in anthocyanin transport in torenia petals, and that anthocyanins and flavones are likely transported to the vacuole through different mechanisms.
谷胱甘肽转移酶（GST）家族Phi类的成员已知在花青素向液泡的转运中起作用。我们从蓝猪耳花瓣cDNA文库中分离出一个GST同源物。从开蓝色花的蓝猪耳品种培育出转录GST双链RNA的转基因植物。这些植物呈现出从蓝色到几乎白色的一系列花色。定量逆转录聚合酶链反应证实了GST转录本的下调，同时转基因植物花瓣中的花青素水平降低，而黄酮水平保持不变。这些结果表明，GST参与蓝猪耳花瓣中的花青素转运，并且花青素和黄酮可能通过不同机制转运到液泡中。
