College of Tea Science and Tea Culture/Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China; Tea Research Institute, Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Hangzhou, 310008, Zhejiang, China.
College of Tea Science and Tea Culture/Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China.
Int J Biol Macromol. 2022 Apr 30;205:749-760. doi: 10.1016/j.ijbiomac.2022.03.109. Epub 2022 Mar 22.
Glutathione S-transferases (GSTs) are ubiquitous enzymes involved in the regulation of plant growth, development, and stress responses. Unfortunately, the comprehensive identification of GSTs in tea plant has not been achieved. In this study, a total of 88 CsGSTs proteins were identified and divided into eight classes, among which the tau class was the largest. Chromosomal localization analysis revealed an uneven distribution of CsGSTs across the tea plant genome. Tandem duplication is the main force driving tea plant CsGSTs expansion. CsGSTs structures and conserved motifs were similar. The analysis of cis-regulatory elements in promoter regions showed that CsGSTs can response to multiple stresses, and that MYB may be involved in the transcriptional regulation of CsGST. RNA-Seq data revealed that the expression of most GSTUs was associated with various stresses, including pathogen and insect attack, cold spells, drought and salt stresses, nitrogen nutrition, bud dormancy, and morphological development, and the expression of these CsGSTs was obviously different in eight tissues. In addition, we proved that CsGSTU19, localized at the nucleus and cell membrane, was involved in tea plant defense against temperature stresses and Co. camelliae infection. These findings provide references for the further functional analysis of GSTs in the future.
谷胱甘肽 S-转移酶（GSTs）是参与植物生长、发育和应激反应调节的普遍存在的酶。不幸的是，茶树 GSTs 的全面鉴定尚未实现。在这项研究中，共鉴定出 88 个 CsGSTs 蛋白，并将其分为 8 个类别，其中 tau 类是最大的。染色体定位分析显示，CsGSTs 在茶树基因组中分布不均匀。串联重复是驱动茶树 CsGSTs 扩张的主要力量。CsGSTs 的结构和保守基序相似。启动子区顺式调控元件的分析表明，CsGSTs 可以对多种胁迫做出反应，而 MYB 可能参与 CsGST 的转录调控。RNA-Seq 数据显示，大多数 GSTUs 的表达与多种胁迫有关，包括病原体和昆虫攻击、寒害、干旱和盐胁迫、氮营养、芽休眠和形态发育，这些 CsGSTs 在 8 种组织中的表达明显不同。此外，我们证明了定位于细胞核和细胞膜的 CsGSTU19 参与了茶树对温度胁迫和 Co. camelliae 感染的防御。这些发现为 GSTs 在未来的功能分析提供了参考。
