Department of Natural Sciences, Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic.
Department of Medical Chemistry and Biomedical Biochemistry, Second Faculty of Medicine and Faculty Hospital Motol, Charles University Prague, Prague, Czech Republic.
PeerJ. 2024 Oct 23;12:e18077. doi: 10.7717/peerj.18077. eCollection 2024.
PAN fibers are characterized by having a large surface-to-volume ratio and small pores, which are beneficial for applications in filtration and specific molecular detection systems. Naturally, larger items are filtered, and a lower ratio between specific and nonspecific binding is expected since small pores do not allow larger elements to penetrate through membranes; thus, nonspecific binding is enhanced. We prepared and tested fiber membranes (diameter cca 700 nm) functionalized with a specific antibody to prove that even microscopic systems such as bacteria could be specifically identified. In addition, we established a methodology that enabled the effective binding of bacteria in not only an aqueous environment but also air. Our data clearly prove that even large systems such as bacteria could be specifically identified by fiber membranes surface-functionalized with a specific antibody. This research opens the door to the construction of biosensors for the fast, inexpensive, and sensitive identification of airborne bacterial contaminants and other airborne pollutants.
PAN 纤维的特点是具有较大的表面积与体积比和较小的孔隙，这有利于在过滤和特定分子检测系统中的应用。较大的物质自然会被过滤掉，并且由于小孔隙不允许较大的元素穿透膜，因此预计特异性和非特异性结合的比例会更低，非特异性结合会增强。我们制备并测试了纤维膜（直径约为 700nm），使其功能化与特定抗体，以证明即使是细菌等微观系统也可以被特异性识别。此外，我们建立了一种方法，使细菌不仅可以在水相环境中，而且可以在空气中有效地结合。我们的数据清楚地证明，即使是像细菌这样的大系统也可以通过表面功能化特定抗体的纤维膜来特异性识别。这项研究为构建用于快速、廉价、敏感地识别空气传播细菌污染物和其他空气污染物的生物传感器开辟了道路。
