Zhou Pei, Xie Si-Qing, Zhong Li-Li, Ding Xiao-Fang
Hunan Provincial Key Laboratory of Pediatric Respiratory Medicine, Children's Medical Center, Hunan Provincial People's Hospital/First Affiliated Hospital of Hunan Normal University, Changsha 410005, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2024 Oct 15;26(10):1115-1121. doi: 10.7499/j.issn.1008-8830.2407088.
To investigate the effects of propranolol on the proliferation, apoptosis, migration, and tube formation ability of human umbilical vein endothelial cells (HUVEC), as well as its impact on the expression of sex-determining region Y-box 18 (SOX18), matrix metalloproteinase-7 (MMP-7), and vascular endothelial growth factor A (VEGFA).
HUVEC were treated with different concentrations of propranolol, and cell viability was assessed using the CCK-8 method to determine the optimal concentration and treatment duration. The experiment consisted of a control group and groups treated with different concentrations of propranolol (50, 100, 150 μmol/L). Apoptosis, migration, and tube formation of HUVEC were observed using flow cytometry, wound healing assays, and tube formation assays. Western blot and real-time quantitative PCR were used to detect the expression levels of SOX18, MMP-7, and VEGFA proteins and mRNA.
Compared to the control group, the apoptosis rate in the propranolol treatment groups increased significantly (<0.05), and it rose significantly with increasing drug concentration (<0.05). The wound healing rate decreased in the propranolol treatment groups, and both the number of tube formation nodes and total tube length were reduced (<0.05). The expression levels of SOX18, MMP-7, and VEGFA proteins and mRNA were downregulated in the propranolol treatment groups (<0.05).
Propranolol can inhibit the proliferation, migration, and tube formation ability of HUVEC and promote cell apoptosis, resulting in decreased expression levels of SOX18, MMP-7, and VEGFA.
探讨普萘洛尔对人脐静脉内皮细胞（HUVEC）增殖、凋亡、迁移及管腔形成能力的影响，以及对性别决定区Y框蛋白18（SOX18）、基质金属蛋白酶-7（MMP-7）和血管内皮生长因子A（VEGFA）表达的影响。
用不同浓度的普萘洛尔处理HUVEC，采用CCK-8法评估细胞活力，以确定最佳浓度和处理时间。实验分为对照组和用不同浓度普萘洛尔（50、100、150μmol/L）处理的组。采用流式细胞术、伤口愈合试验和管腔形成试验观察HUVEC的凋亡、迁移和管腔形成情况。采用蛋白质免疫印迹法和实时定量PCR检测SOX18、MMP-7和VEGFA蛋白及mRNA的表达水平。
与对照组相比，普萘洛尔处理组的凋亡率显著升高（<0.05），且随药物浓度增加而显著升高（<0.05）。普萘洛尔处理组的伤口愈合率降低，管腔形成节点数和总管长度均减少（<0.05）。普萘洛尔处理组中SOX18、MMP-7和VEGFA蛋白及mRNA表达水平下调（<0.05）。
普萘洛尔可抑制HUVEC的增殖、迁移和管腔形成能力，促进细胞凋亡，导致SOX18、MMP-7和VEGFA表达水平降低。
