Tang Luyao, Lian Jinjin, Zhao Beibei, Shen Zhiqi, Zhu Zhenan, Ye Yuhan, Zhang Zhining, Lu Mei, Wang Yuexing, Rao Yuchun
College of Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China.
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Oct 25;40(10):3485-3499. doi: 10.13345/j.cjb.240357.
Vitamin B6 (VB6), as an essential component involved in numerous biological activities of animals and plants, reflects the nutritional quality of cereal crops such as rice. Few studies have been conducted to mine the genes controlling the VB6 content in rice grains, and the available studies remain to be deepened. In this study, the recombinant inbred lines created from parents 'HZ' and 'Nekken2' served as the experimental materials. Based on QTL mapping, the initial screening identified ten candidate genes. The expression levels of _, _, _, _, _, and _ showed significant differences between the parents. According to the gene expression and parental VB6 content, we hypothesized _ as the key gene affecting the VB6 content in rice grains, and the high expression of this gene significantly influenced the VB6 content. The results of this study fill a gap in the QTL mapping on the VB6 content of rice grains and provide theoretical support for elucidating the molecular genetic mechanisms and cloning the related genes of VB6 synthesis in rice. In addition, the findings have significant implications for identifying, screening, and breeding new rice cultivars with high VB6 content.
维生素B6（VB6）作为参与动植物众多生物活动的必需成分，反映了水稻等谷类作物的营养品质。目前挖掘控制水稻籽粒中VB6含量基因的研究较少，现有研究仍有待深入。本研究以亲本“HZ”和“Nekken2”构建的重组自交系为实验材料。基于QTL定位，初步筛选出10个候选基因。其中_、、、_、_和_的表达水平在亲本间存在显著差异。根据基因表达情况和亲本的VB6含量，我们推测_是影响水稻籽粒VB6含量的关键基因，该基因的高表达显著影响了VB6含量。本研究结果填补了水稻籽粒VB6含量QTL定位的空白，为阐明水稻VB6合成的分子遗传机制及克隆相关基因提供了理论支持。此外，这些发现对于鉴定、筛选和培育高VB6含量的水稻新品种具有重要意义。
