Department of Infectious Diseases, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.
Wuhan Research Center for Infectious Diseases and Cancer, Chinese Academy of Medical Sciences, Wuhan, Hubei, China.
BMC Infect Dis. 2024 Oct 28;24(1):1210. doi: 10.1186/s12879-024-10009-8.
Information on the comparison of blood microbiota between human immunodeficiency virus (HIV)-infected and HIV-uninfected patients with suspected sepsis by metagenomic next-generation sequencing (mNGS) is limited.
Retrospectively analysis was conducted in HIV-infected and HIV-uninfected patients with suspected sepsis at Changsha First Hospital (China) from March 2019 to August 2022. Patients who underwent blood mNGS testing were enrolled. The blood microbiota detected by mNGS were analyzed.
A total of 233 patients with suspected sepsis who performed blood mNGS were recruited in this study, including 79 HIV-infected and 154 HIV-uninfected patients. Compared with HIV-uninfected patients, the proportions of mycobacterium (p = 0.001), fungus (p < 0.001) and viruses (p < 0.001) were significantly higher, while the proportion of bacteria (p = 0.001) was significantly lower in HIV-infected patients. The higher positive rates of non-tuberculous mycobacteriosis (NTM, p = 0.022), Pneumocystis jirovecii (P. jirovecii) (p = 0.014), Talaromyces marneffei (T. marneffei) (p < 0.001) and cytomegalovirus (CMV) (p < 0.001) were observed in HIV-infected patients, compared with HIV-uninfected patients. In addition, compared with HIV-uninfected patients, the constituent ratio of T. marneffei (p < 0.001) in the fungus spectrum were significantly higher, while the constituent ratios of Candida (p < 0.001) and Aspergillus (p = 0.001) were significantly lower in HIV-infected patients.
Significant differences in the blood microbiota profiles exist between HIV-infected and HIV-uninfected patients with suspected sepsis.
通过宏基因组下一代测序（mNGS）比较人类免疫缺陷病毒（HIV）感染和未感染疑似脓毒症患者的血液微生物组的信息有限。
回顾性分析 2019 年 3 月至 2022 年 8 月在中国长沙市第一医院就诊的 HIV 感染和未感染疑似脓毒症患者。招募接受血液 mNGS 检测的患者。分析 mNGS 检测到的血液微生物组。
本研究共纳入 233 例接受疑似脓毒症血液 mNGS 检测的患者，其中包括 79 例 HIV 感染患者和 154 例 HIV 未感染患者。与 HIV 未感染患者相比，HIV 感染患者的分枝杆菌（p=0.001）、真菌（p<0.001）和病毒（p<0.001）的比例显著更高，而细菌（p=0.001）的比例显著更低。非结核分枝杆菌病（NTM，p=0.022）、卡氏肺孢子虫（P. jirovecii，p=0.014）、马尔尼菲青霉（T. marneffei，p<0.001）和巨细胞病毒（CMV，p<0.001）的阳性率在 HIV 感染患者中显著更高。此外，与 HIV 未感染患者相比，HIV 感染患者的真菌谱中 T. marneffei 的构成比显著更高（p<0.001），而 Candida（p<0.001）和 Aspergillus（p=0.001）的构成比显著更低。
疑似脓毒症的 HIV 感染和未感染患者的血液微生物组谱存在显著差异。
