College of Agronomy, Gansu Agricultural University, Lanzhou, China.
College of Life Science and Technology, Gansu Agricultural University, Lanzhou, China.
Physiol Plant. 2024 Nov-Dec;176(6):e14578. doi: 10.1111/ppl.14578.
SNF1-RELATED KINASE 2 (SnRK2) plays a crucial role in plants' stress response. Although studies have reported that the overexpression of several SnRK2 family members in different plants leads to improved stress tolerance, it is difficult to elucidate the mechanisms by which SnRK2s regulate stress tolerance due to the variability of experimental variables in these studies. Therefore, we used meta-analysis to comprehensively analyze 22 parameters that can reflect drought tolerance and salinity tolerance in SnRK2s-transformed plants and to explore the effects that different experimental variables between studies have on the relevant plant parameters. The results showed that the overexpression of SnRK2s mainly improved plants' drought and salinity tolerance by reducing their osmotic stress and oxidative damage, improving photosynthesis and other biochemical and physiological processes. Out of the 22 physiological parameters, 17 and 19 were significantly affected by drought and salt stress, respectively, and 10 indicators were also significantly changed under non-stress conditions. Under salt stress, the cell membrane permeability among these parameters shows the most significant changes, increasing by 506.57% in SnRK2-overexpressing plants compared to wild type (WT). Therefore, although plants overexpressing SnRK2s respond positively to both drought and salt stress, they demonstrated greater tolerance to salt stress. In addition, among the detected regulatory variables, donor-acceptor type, promoter type, stress type, experimental medium, and duration all affected the extent of SnRK2s overexpression and affected the physiological characteristics of the transgenic plants. Also, different stress conditions (salt, drought stress) led to different degrees of transformation. These studies provide new research directions for studying crop stress tolerance and help to better explore the functions played by SnRK2s in external plant stresses.
SNF1-RELATED KINASE 2（SnRK2）在植物的应激反应中起着至关重要的作用。虽然研究报道，在不同的植物中过度表达几个 SnRK2 家族成员会导致对胁迫的耐受性提高，但由于这些研究中实验变量的可变性，很难阐明 SnRK2 调节胁迫耐受性的机制。因此，我们使用荟萃分析综合分析了 22 个参数，这些参数可以反映 SnRK2 转化植物的耐旱性和耐盐性，并探讨了研究之间不同实验变量对相关植物参数的影响。结果表明，SnRK2 的过表达主要通过降低渗透胁迫和氧化损伤，改善光合作用和其他生化和生理过程来提高植物的耐旱性和耐盐性。在 22 个生理参数中，有 17 个和 19 个分别受干旱和盐胁迫的显著影响，10 个指标在非胁迫条件下也发生了显著变化。在盐胁迫下，这些参数中细胞膜通透性的变化最为显著，SnRK2 过表达植株比野生型（WT）增加了 506.57%。因此，尽管过表达 SnRK2 的植物对干旱和盐胁迫均有积极响应，但它们对盐胁迫的耐受性更强。此外，在检测到的调节变量中，供体-受体类型、启动子类型、胁迫类型、实验介质和持续时间都影响 SnRK2 的过表达程度，并影响转基因植物的生理特征。此外，不同的胁迫条件（盐、干旱胁迫）导致转化程度不同。这些研究为研究作物胁迫耐受性提供了新的研究方向，并有助于更好地探索 SnRK2 在植物外部胁迫中的功能。
