（Miq.）Koidz.对顺铂诱导的大鼠的肾保护活性：降低血清肌酐、尿素氮、KIM-1，并抑制 NF-κB p65 和 COX-2。
Nephroprotective Activity of (Miq). Koidz. on Cisplatin-Induced Rats: Reducing Serum Creatinine, Urea Nitrogen, KIM-1, and Suppressing NF-kappaB p65 and COX-2.
机构信息
Master Program in Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, West Java, Indonesia.
Faculty of Health, Universitas Nahdlatul Ulama, Mataram, West Nusa Tenggara, Indonesia.
出版信息
Drug Des Devel Ther. 2024 Oct 24;18:4707-4721. doi: 10.2147/DDDT.S481479. eCollection 2024.
BACKGROUND
The sap of (Miq). Koidz. has been reported for its abundance of chalcone contents. Chalcones have been known for their effective nephroprotective activity toward cisplatin-induced renal cells and mice.
PURPOSE
To investigate the effect of sap extract (ASEE) on kidney function parameters (serum creatinine, urea nitrogen, and kidney injury molecule-1) and the expression of NF-kappaB p65 and COX-2 in cisplatin-induced Wistar rats.
METHODS
In vivo nephroprotective activity of ASEE at 1000 and 1500 mg/kg BW/day doses for 10 days on cisplatin (5 mg/kg BW) induced nephrotoxicity was evaluated on Wistar rats. Quercetin 20 mg/kg BW/day was used as the control drug. Cisplatin inducement was given on day 7. The BW was measured every day. On day 11, the rats were euthanized, and their blood was taken intracardially for creatinine and urea nitrogen analysis. Histopathological analysis was carried out on the right kidney, and KIM-1 levels in the left kidney were measured. The Western blot technique evaluated the NF-kappaB p65 and COX-2 expression in the kidney. All data obtained were compared to the cisplatin group (negative control). The total flavonoids and chalcones in ASEE were also determined.
RESULTS
Pretreatment with ASEE reduces the BW of Wistar rats, and significantly reduces creatinine and KIM-1 levels, but does not significantly reduce the levels of urea nitrogen, the expression of NF-kappaB p65, and COX-2 in the kidney of cisplatin-induced Wistar rats. The total flavonoid content in ASEE is 8.755 g QE/100 g extract and the total chalcone content is 5.532 g IBCE/100 g extract.
CONCLUSION
The sap of (Miq). Koidz. reveal the potential to protect the kidneys against cisplatin-induced toxicity. The nephroprotective activity may be attributed to the antioxidant and anti-inflammatory properties of the flavonoids and the chalcones contained in the sap of (Miq). Koidz.
背景
已报道（Miq.）Koidz. 的树液中含有丰富的查尔酮类物质。查尔酮类物质已被证实具有有效的肾保护活性，可对抗顺铂诱导的肾细胞和小鼠的损伤。
目的
研究树液提取物（ASEE）对顺铂诱导的 Wistar 大鼠肾功能参数（血清肌酐、尿素氮和肾损伤分子-1）以及 NF-κB p65 和 COX-2 表达的影响。
方法
将 Wistar 大鼠连续 10 天每天分别给予 1000 和 1500 mg/kg BW 的 ASEE 剂量以及 20 mg/kg BW 的阳性对照药 quercetin，以评估 ASEE 在 5 mg/kg BW 的顺铂诱导的肾毒性中的体内肾保护活性。顺铂诱导在第 7 天进行。每天测量 BW。第 11 天，大鼠安乐死后心脏取血，用于肌酐和尿素氮分析。对右肾进行组织病理学分析，并测量左肾 KIM-1 水平。Western blot 技术评估了肾组织中 NF-κB p65 和 COX-2 的表达。将所有获得的数据与顺铂组（阴性对照）进行比较。还测定了 ASEE 中的总类黄酮和查尔酮。
结果
ASEE 预处理可降低 Wistar 大鼠的 BW，并显著降低肌酐和 KIM-1 水平，但对顺铂诱导的 Wistar 大鼠肾组织中尿素氮、NF-κB p65 和 COX-2 的表达无显著影响。ASEE 中的总类黄酮含量为 8.755 g QE/100 g 提取物，总查尔酮含量为 5.532 g IBCE/100 g 提取物。
结论
（Miq.）Koidz. 的树液具有保护肾脏免受顺铂诱导的毒性的潜力。肾保护活性可能归因于树液中所含的类黄酮和查尔酮的抗氧化和抗炎特性。
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