The Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, China.
Research Base of Traditional Chinese Medicine Syndrome, Fujian University of Traditional Chinese Medicine, Fuzhou, China.
Medicine (Baltimore). 2024 Oct 25;103(43):e40093. doi: 10.1097/MD.0000000000040093.
Erectile dysfunction (ED) is a common male sexual health problem that can be associated with obesity. This study aimed to identify serum metabolic differences and pathways related to ED in obese men using non-targeted metabolomics techniques. We included 54 obese male patients with (n = 27) and without (n = 27) ED. We collected 5 mL of fasting elbow vein blood and analyzed serum metabolites using ultra-high-performance liquid chromatography-mass spectrometry. Multivariate statistical methods (principal component analysis and orthogonal partial least squares discriminant analysis) were used to identify differential metabolites between the groups. Finally, pathway analysis using the Kyoto encyclopedia of genes and genomes database identified 4 differential metabolic pathways in obese men with ED compared to obese men without ED. A total of 77 differential metabolites were identified in obese men with ED compared to the control group (obese men without ED) using a threshold of variable importance in the projection > 1 and P < .05. Pathway analysis revealed 4 main differences: glycine, serine and threonine metabolism, glycerophospholipid metabolism, aminoacyl-tRNA biosynthesis, and D-glutamine and D-glutamate metabolism. Specific metabolites associated with these pathways included betaine aldehyde, choline, L-threonine, phosphatidylcholine, L-serine, and D-glutamine. Our findings suggest abnormalities in fatty acid metabolism, phospholipid metabolism, and amino acid metabolism between obese men with and without ED. Metabolites such as betaine aldehyde, choline, L-threonine, phosphatidylcholine, L-serine, and D-glutamine may be potential biomarkers for distinguishing obese men with ED.
勃起功能障碍（ED）是一种常见的男性性功能健康问题，可能与肥胖有关。本研究旨在使用非靶向代谢组学技术，鉴定与肥胖男性 ED 相关的血清代谢差异和途径。我们纳入了 54 名肥胖男性患者，其中 27 名患有 ED（病例组），27 名无 ED（对照组）。采集空腹肘静脉血 5ml，采用超高效液相色谱-质谱联用技术分析血清代谢物。采用主成分分析和正交偏最小二乘判别分析等多变量统计方法鉴定组间差异代谢物。最后，京都基因与基因组百科全书数据库的通路分析鉴定出 ED 肥胖男性与非 ED 肥胖男性相比存在 4 个差异代谢通路。ED 肥胖男性与对照组（非 ED 肥胖男性）相比，共鉴定出 77 个差异代谢物（阈值为投影重要性>1 和 P<0.05）。通路分析显示，ED 肥胖男性存在 4 个主要差异：甘氨酸、丝氨酸和苏氨酸代谢、甘油磷脂代谢、氨酰-tRNA 生物合成以及 D-谷氨酰胺和 D-谷氨酸代谢。与这些通路相关的特定代谢物包括甜菜醛、胆碱、L-苏氨酸、磷脂酰胆碱、L-丝氨酸和 D-谷氨酰胺。我们的研究结果表明，肥胖男性中 ED 与非 ED 之间存在脂肪酸代谢、磷脂代谢和氨基酸代谢异常。甜菜醛、胆碱、L-苏氨酸、磷脂酰胆碱、L-丝氨酸和 D-谷氨酰胺等代谢物可能是鉴别 ED 肥胖男性的潜在生物标志物。
