Department of Hydraulics and Sanitation, São Carlos School of Engineering, University of São Paulo, Av. João Dagnone, 1100 - Jd. Santa Angelina, São Carlos, SP, 13563-120, Brazil.
Bioenergy Research Institute (IPBEN), UNESP- São Paulo State University, Rio Claro, SP, 13500-230, Brazil.
Biodegradation. 2024 Oct 29;36(1):3. doi: 10.1007/s10532-024-10097-y.
The study evaluated the performance of thermophilic co-digestion in both single-stage methanogenic reactors (TMR) and two-stage systems, consisting of a thermophilic acidogenic reactor and a thermophilic sequential methanogenic reactor (TSMR). A 1:1 mixture of sugarcane vinasse and molasses was codigested in anaerobic fluidized bed reactors, with varying organic matter concentrations based on chemical oxygen demand (COD) ranging from 5 to 22.5 g COD L. Both systems achieved high organic matter removal efficiency (51 to 86.5%) and similar methane (CH) yields (> 148 mL CH gCOD). However, at the highest substrate concentration (22.5 g COD L), the TSMR outperformed the TMR in terms of energy generation potential (205.6 kJ d vs. 125 kJ d). Phase separation in the two-stage system increased bioenergy generation by up to 43.5% at lower substrate concentrations (7.5 g COD L), with hydrogen (H) generation playing a critical role in this enhancement. Additionally, the two-stage system produced value-added products, including ethanol (2.3 g L), volatile organic acids (3.2 g lactate L), and H (0.6-2.7 L H L d). Microbial analysis revealed that Thermoanaerobacterium, Caldanaerobius, and Clostridium were dominant at 5 g COD L, while Lactobacillus prevailed at concentrations of ≥ 15 g COD L. The primary methane producers in the single-stage system were Methanosarcina, Methanoculleus, and Methanobacterium, whereas Methanothermobacter, Bathyarchaeia, and Methanosarcina dominated in the two-stage system.
该研究评估了嗜热协同消化在单级产甲烷反应器（TMR）和两级系统中的性能，两级系统由一个嗜热产酸反应器和一个嗜热顺序产甲烷反应器（TSMR）组成。在厌氧流化床反应器中，以甘蔗废酒糟和糖蜜 1:1 的混合物为原料，基于化学需氧量（COD）的不同有机物浓度范围为 5 至 22.5 g COD L。两个系统均实现了高有机物去除效率（51%至 86.5%）和相似的甲烷（CH）产率（>148 mL CH gCOD）。然而，在最高底物浓度（22.5 g COD L）下，TSMR 在能源产生潜力方面优于 TMR（205.6 kJ d 对 125 kJ d）。两相系统中的相分离在较低的底物浓度（7.5 g COD L）下可将生物能源产生提高多达 43.5%，其中氢气（H）的产生在这种增强中起着关键作用。此外，两相系统还产生了增值产品，包括乙醇（2.3 g L）、挥发性有机酸（3.2 g 乳酸 L）和 H（0.6-2.7 L H L d）。微生物分析表明，在 5 g COD L 时，Thermoanaerobacterium、Caldanaerobius 和 Clostridium 占主导地位，而在浓度≥15 g COD L 时，Lactobacillus 占主导地位。在单级系统中，主要的甲烷产生菌是 Methanosarcina、Methanoculleus 和 Methanobacterium，而在两级系统中，主要的甲烷产生菌是 Methanothermobacter、Bathyarchaeia 和 Methanosarcina。
