Ribeiro Alexandre Rodrigues, Devens Kauanna Uyara, Camargo Franciele Pereira, Sakamoto Isabel Kimiko, Varesche Maria Bernadete Amâncio, Silva Edson Luiz
Department of Hydraulics and Sanitation, São Carlos School of Engineering, University of São Paulo, Av. João Dagnone, 1100 - Jd. Santa Angelina, São Carlos, SP, CEP 13563-120, Brazil.
Bioenergy Research Institute (IPBEN), UNESP- São Paulo State University, Rio Claro, SP, 13500-230, Brazil.
Appl Biochem Biotechnol. 2025 Feb;197(2):964-988. doi: 10.1007/s12010-024-05078-z. Epub 2024 Sep 28.
This study assessed the impact of organic loading rate (OLR) on methane (CH) production in the anaerobic co-digestion (AcoD) of sugarcane vinasse and molasses (SVM) (1:1 ratio) within a thermophilic fluidized bed reactor (AFBR). The OLR ranged from 5 to 27.5 kg COD.m.d, with a fixed hydraulic retention time (HRT) of 24 h. Organic matter removal varied from 56 to 84%, peaking at an OLR of 5 kg COD.m.d. Maximum CH yield (MY) (272.6 mL CH.gCOD) occurred at an OLR of 7.5 kg COD.m.d, while the highest CH production rate (MPR) (4.0 L CH.L.d) and energy potential (E.P.) (250.5 kJ.d) were observed at an OLR of 20 kg COD.m.d. The AFBR exhibited stability across all OLR. At 22.5 kg COD.m.d, a decrease in MY indicated methanogenesis imbalance and inhibitory organic compound accumulation. OLR influenced microbial populations, with Firmicutes and Thermotogota constituting 43.9% at 7.5 kg COD.m.d, and Firmicutes dominating (52.7%) at 27.5 kg COD.m.d. Methanosarcina (38.9%) and hydrogenotrophic Methanothermobacter (37.6%) were the prevalent archaea at 7.5 kg COD.m.d and 27.5 kg COD.m.d, respectively. Therefore, this study demonstrates that the organic loading rate significantly influences the efficiency of methane production and the stability of microbial communities during the anaerobic co-digestion of sugarcane vinasse and molasses, indicating that optimized conditions can maximize energy yield and maintain methanogenic balance.
本研究评估了有机负荷率（OLR）对嗜热流化床反应器（AFBR）中甘蔗酒糟和糖蜜（SVM）（1:1比例）厌氧共消化过程中甲烷（CH）产生的影响。OLR范围为5至27.5 kg COD·m⁻³·d⁻¹，固定水力停留时间（HRT）为24小时。有机物去除率在56%至84%之间变化，在OLR为5 kg COD·m⁻³·d⁻¹时达到峰值。最大甲烷产量（MY）（272.6 mL CH₄·gCOD⁻¹）出现在OLR为7.5 kg COD·m⁻³·d⁻¹时，而最高甲烷生产率（MPR）（4.0 L CH₄·L⁻¹·d⁻¹）和能量潜力（E.P.）（250.5 kJ·d⁻¹）在OLR为20 kg COD·m⁻³·d⁻¹时观察到。AFBR在所有OLR下均表现出稳定性。在22.5 kg COD·m⁻³·d⁻¹时，MY的下降表明甲烷生成失衡和抑制性有机化合物积累。OLR影响微生物种群，在OLR为7.5 kg COD·m⁻³·d⁻¹时，厚壁菌门和栖热袍菌门占43.9%，在OLR为27.5 kg COD·m⁻³·d⁻¹时厚壁菌门占主导（52.7%）。在OLR为7.5 kg COD·m⁻³·d⁻¹和27.5 kg COD·m⁻³·d⁻¹时，甲烷八叠球菌（38.9%）和氢营养型嗜热栖热菌（37.6%）分别是主要的古菌。因此，本研究表明，在甘蔗酒糟和糖蜜的厌氧共消化过程中，有机负荷率显著影响甲烷产生效率和微生物群落的稳定性，这表明优化条件可以使能量产量最大化并维持甲烷生成平衡。
