Mohammadi Zahra, Rahaie Mahdi, Moradifar Fatemeh
Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, 14399-57131, Iran.
J Fluoresc. 2024 Oct 29. doi: 10.1007/s10895-024-03998-x.
Glyphosate has become the most widely used herbicide worldwide in recent years. There are many concerns about toxicity and mutagenicity from long-term use of glyphosate in humans and animals. Therefore, the methods that can help in easy and quick detection of this chemical compound in food and water are critical. In this work, a biosensor was fabricated by combining the enzymatic properties of a specific DNA G-quadruplex and selectivity of a split aptamer to detect glyphosate in foods and water in a quick and simple colorimetric manner. The color change in this method is based on the oxidation of TMB by the G-quadruplex enzyme and the function of aptamer to trap glyphosate, which is visible to the naked eye in the presence and absence of the herbicide. The biosensor showed its high performance in various real samples of water and foods and provided a detection limit of 1.37 nM (R² = 0.9899) with a linear response range of 100 to 400 nM of glyphosate. This biosensor can provide an innovative, cheap and fast approach for the detection and monitoring of glyphosate in various foods and water.
近年来，草甘膦已成为全球使用最广泛的除草剂。长期使用草甘膦对人和动物的毒性及致突变性引发了诸多担忧。因此，能够有助于简便快速检测食品和水中这种化合物的方法至关重要。在这项工作中，通过结合特定DNA G-四链体的酶促特性和分裂适体的选择性，构建了一种生物传感器，以快速简便的比色法检测食品和水中的草甘膦。该方法中的颜色变化基于G-四链体酶对TMB的氧化以及适体捕获草甘膦的功能，在有和没有除草剂的情况下肉眼均可观察到。该生物传感器在各种水和食品实际样品中表现出高性能，对草甘膦的检测限为1.37 nM（R² = 0.9899），线性响应范围为100至400 nM。这种生物传感器可为检测和监测各种食品和水中的草甘膦提供一种创新、廉价且快速的方法。
