Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Asian Pac J Cancer Prev. 2024 Oct 1;25(10):3507-3513. doi: 10.31557/APJCP.2024.25.10.3507.
To investigate the relationship between Yes-Associated Protein (YAP) and Vascular Endothelial Growth Factor-A (VEGF-A) in nasopharyngeal carcinoma (NPC) and evaluate their potential as predictive biomarkers for distant metastasis.
This observational analytic study was conducted from May to August 2023, including 60 NPC cases diagnosed histopathologically at the Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo Hospital. The study involved 30 NPC cases with distant metastasis and 30 without distant metastasis. Immunohistochemical analysis was performed using primary antibodies (rabbit YAP, D8H1X; mouse VEGF, clone C-1 sc-7269). Expression levels of YAP and VEGF-A were semi-quantitatively assessed using the H-score formula on 500 tumor cells. Cutoff points for YAP and VEGF-A were determined through Receiver Operating Characteristic (ROC) curve analysis and the Youden index. Statistical analyses, including Chi-square tests, were conducted using SPSS 25.0 to evaluate the relationship between marker expression and the presence of distant metastasis.
A significant difference in YAP expression was observed between NPC cases with distant metastasis (80%) and those without (23.3%, p<0.001). Similarly, VEGF-A expression was notably higher in cases with distant metastasis (86.7%) compared to those without (20%, p<0.001). A significant association between YAP and VEGF-A expression was found (p=0.001). ROC curve analysis showed that YAP (AUC=0.738, 95% CI 0.602-0.874) and VEGF-A (AUC=0.842, 95% CI 0.735-0.950) effectively predict distant metastasis. The double co-high-expression group (elevated YAP and VEGF-A) exhibited a significantly higher rate of distant metastasis compared to the non-double co-high-expression group (p<0.001).
Elevated expressions of YAP and VEGF-A are significantly associated with distant metastasis in NPC, suggesting their potential as biomarkers for predicting metastatic risk.
探讨 Yes 相关蛋白（YAP）与血管内皮生长因子 A（VEGF-A）在鼻咽癌（NPC）中的关系，并评估它们作为远处转移预测生物标志物的潜力。
本观察性分析研究于 2023 年 5 月至 8 月在印度尼西亚大学医学院解剖病理学系进行，纳入 60 例经组织病理学诊断的 NPC 病例。研究包括 30 例远处转移 NPC 病例和 30 例无远处转移 NPC 病例。采用兔 YAP（D8H1X）和鼠 VEGF（clone C-1 sc-7269）单克隆抗体进行免疫组织化学分析。采用 H 评分公式对 500 个肿瘤细胞进行 YAP 和 VEGF-A 的半定量评估。通过受试者工作特征（ROC）曲线分析和 Youden 指数确定 YAP 和 VEGF-A 的截断值。采用 SPSS 25.0 进行统计分析，包括卡方检验，以评估标记物表达与远处转移存在之间的关系。
有远处转移的 NPC 病例中 YAP 表达（80%）明显高于无远处转移的病例（23.3%，p<0.001）。同样，有远处转移的病例中 VEGF-A 表达（86.7%）明显高于无远处转移的病例（20%，p<0.001）。YAP 和 VEGF-A 表达之间存在显著相关性（p=0.001）。ROC 曲线分析显示，YAP（AUC=0.738，95%CI 0.602-0.874）和 VEGF-A（AUC=0.842，95%CI 0.735-0.950）可有效预测远处转移。双高表达组（YAP 和 VEGF-A 均升高）远处转移率明显高于非双高表达组（p<0.001）。
YAP 和 VEGF-A 的高表达与 NPC 远处转移显著相关，提示其作为预测转移风险的生物标志物的潜力。
