Alnukhba University Collage, Medical Laboratory Department, Baghdad, Iraq.
Al-Iraqia University, College of Medicine, Medical Microbiology Department, Baghdad, Iraq.
Asian Pac J Cancer Prev. 2024 Oct 1;25(10):3701-3705. doi: 10.31557/APJCP.2024.25.10.3701.
This study investigated the cytotoxic effects of Stenotrophomonas maltophilia on normal human cells.
Seven isolates of S. maltophilia were obtained from 120 urine samples collected from prostate and bladder cancer patients, with diagnoses confirmed via Vitek. The cytotoxicity of these bacterial isolates was assessed on normal human fibrocyte cells (NHF) using various concentrations of bacterial filtrate (3.125, 6.25, 12.5, 25, 50, and 100 μg/ml).
The bacterial filtrate exhibited significant toxicity to NHF cells, with the highest cell death rate of 68% and the lowest optical density (OD) of 0.25 at a concentration of 100 μg/ml. The IC50 value, indicating the concentration at which 50% inhibition of cell viability occurred, was determined to be 49.35 μg/ml. Further research is necessary to explore the potential role of these bacterial isolates in promoting cancer through inflammation.
The clinical isolates of S. maltophilia demonstrated substantial cytotoxic activity against normal human fibrocyte cells, leading to a notable reduction in both cell number and optical density, with the highest percentage of cell death observed. These findings suggest the need for further investigation into the specific toxins or enzymes involved, which could pave the way for future studies.
本研究旨在探讨嗜麦芽寡养单胞菌对正常人体细胞的细胞毒性作用。
从经 Vitek 确诊的前列腺癌和膀胱癌患者的 120 份尿液样本中获得了 7 株嗜麦芽寡养单胞菌。使用不同浓度的细菌滤液（3.125、6.25、12.5、25、50 和 100μg/ml）评估这些细菌分离物对正常人类成纤维细胞（NHF）的细胞毒性。
细菌滤液对 NHF 细胞表现出显著的毒性，在浓度为 100μg/ml 时，细胞死亡率最高为 68%，光密度（OD）最低为 0.25。IC50 值表示细胞活力抑制 50%时的浓度，确定为 49.35μg/ml。需要进一步研究这些细菌分离物是否通过炎症促进癌症的潜在作用。
嗜麦芽寡养单胞菌的临床分离株对正常人类成纤维细胞表现出显著的细胞毒性活性，导致细胞数量和光密度明显减少，观察到的细胞死亡率最高。这些发现表明需要进一步研究涉及的特定毒素或酶，为未来的研究铺平道路。
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