Wang Xin-Tong, Liu Kou-Han, Li Ying, Ren Yan-Yan, Li Qiang, Wang Bao-Tong
State Key Laboratory of Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Shannxi Province, 712100, People's Republic of China.
Stress Biol. 2024 Oct 30;4(1):45. doi: 10.1007/s44154-024-00171-z.
Metalloproteinases are ubiquitous in organisms. Most metalloproteinases secreted by pathogenic microorganisms are also called virulence factors, because they degrade proteins in the external tissues of the host, thereby reducing the host's immunity and increasing its susceptibility to disease. Zinc metalloproteinase is one of the most common metalloproteinases. In our report, we studied the biological function of zinc metalloprotease FgM35 in Fusarium graminearum and the pathogen-host interaction during infection. We found that the asexual and sexual reproduction of the deletion mutant ΔFgM35 were affected, as well as the tolerance of F. graminearum to metal stress. In addition, deletion of FgM35 reduced the virulence of F. graminearum. The wheat target TaZnBP was screened using a wheat yeast cDNA library, and the interaction between FgM35 and TaZnBP was verified by HADDOCK molecular docking, yeast two-hybrid, Bi-FC, Luc, and Co-IP assays. The contribution of TaZnBP to plant immunity was also demonstrated. In summary, our work revealed the indispensable role of FgM35 in the reproductive process and the pathogenicity of F. graminearum, and it identified the interaction between FgM35 and TaZnBP as well as the function of TaZnBP. This provides a theoretical basis for further study of the function of metalloproteinases in pathogen-host interactions.
金属蛋白酶在生物体内普遍存在。大多数病原微生物分泌的金属蛋白酶也被称为毒力因子，因为它们会降解宿主外部组织中的蛋白质，从而降低宿主的免疫力并增加其对疾病的易感性。锌金属蛋白酶是最常见的金属蛋白酶之一。在我们的报告中，我们研究了禾谷镰刀菌中锌金属蛋白酶FgM35的生物学功能以及感染过程中的病原菌 - 宿主相互作用。我们发现缺失突变体ΔFgM35的无性和有性繁殖受到影响，以及禾谷镰刀菌对金属胁迫的耐受性。此外，FgM35的缺失降低了禾谷镰刀菌的毒力。使用小麦酵母cDNA文库筛选了小麦靶标TaZnBP，并通过HADDOCK分子对接、酵母双杂交、双分子荧光互补、荧光素酶互补成像和免疫共沉淀实验验证了FgM35与TaZnBP之间的相互作用。还证明了TaZnBP对植物免疫的贡献。总之，我们的工作揭示了FgM35在禾谷镰刀菌的生殖过程和致病性中不可或缺的作用，并确定了FgM35与TaZnBP之间的相互作用以及TaZnBP的功能。这为进一步研究金属蛋白酶在病原菌 - 宿主相互作用中的功能提供了理论基础。
