分离、鉴定及噬菌体 PAS7、PAS59 和 PAS61 对产志贺毒素大肠杆菌 O103 和 O146 的受体结合蛋白特异性。

Isolation, characterization, and receptor-binding protein specificity of phages PAS7, PAS59 and PAS61 infecting Shiga toxin-producing Escherichia coli O103 and O146.

机构信息

Department of Biotechnology, Ghent University, Valentin Vaerwyckweg 1, Gent, 9000, Belgium.

Food Microbiology Research Group, Institute of Food and Beverage Innovation, Zurich University of Applied Sciences (ZHAW), Einsiedlerstrasse 35, Wädenswil, 8820, Switzerland.

出版信息

Sci Rep. 2024 Oct 30;14(1):26050. doi: 10.1038/s41598-024-77463-x.

DOI:10.1038/s41598-024-77463-x

PMID:39472643

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11522302/

Abstract

Shiga toxin-producing Escherichia coli (STEC) is a foodborne pathogen with 6,534 annual reported cases in the EU in 2021. This pathotype generally contains strains with smooth LPS with O-antigen serogroup O157 being the predominant serogroup in the US. However, non-O157 STEC serogroups are becoming increasingly prevalent. Here we announce the complete genomes of three newly isolated phages that infect STEC serogroups O103 and O146, namely Escherichia phages vB_EcoP_PAS7, vB_EcoP_PAS59 and vB_EcoP_PAS61. The genome sequences revealed that they belong to three distinct genera, namely the newly proposed genus Cepavirus within the Slopekvirinae subfamily, the genus Suseptimavirus and the genus Uetakevirus, respectively. We identified the tailspikes of phages PAS7 and PAS61 as a primary specificity determinant for the O-antigens O103 and O146, respectively, and predicted their active site in silico.

摘要

产志贺毒素大肠杆菌（STEC）是一种食源性病原体，2021 年在欧盟每年报告的病例有 6534 例。该病原体通常包含具有光滑 LPS 的菌株，O 抗原血清群 O157 是美国的主要血清群。然而，非 O157 STEC 血清群的流行程度正在不断增加。在这里，我们宣布了三种新分离的噬菌体的完整基因组，它们感染 STEC 血清群 O103 和 O146，即大肠杆菌噬菌体 vB_EcoP_PAS7、vB_EcoP_PAS59 和 vB_EcoP_PAS61。基因组序列表明，它们属于三个不同的属，即 Slopekvirinae 亚科中新提出的 Cepavirus 属、Suseptimavirus 属和 Uetakevirus 属。我们确定了噬菌体 PAS7 和 PAS61 的尾刺分别是 O 抗原 O103 和 O146 的主要特异性决定因素，并在计算机上预测了它们的活性位点。