Kübler Ines C, Kretzschmar Jenny, Arredondo-Lasso Maria Nieves, Keeley Sean D, Rößler Luca Claudia, Ganss Katharina, Sandoval-Guzmán Tatiana, Brankatschk Marko
Department of Internal Medicine III, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
Biotechnology Center (BIOTEC), Technische Universität Dresden, Dresden, Germany.
NPJ Regen Med. 2024 Oct 29;9(1):33. doi: 10.1038/s41536-024-00375-x.
In regenerating tissues, synthesis and remodeling of membranes rely on lipid turnover and transport. Our study addresses lipid adaptations in intestinal regeneration of Drosophila melanogaster and limb regeneration of Ambystoma mexicanum. We found changes in lipid profiles at different locations: transport, storage organs and regenerating tissues. We demonstrate that attenuating insulin signaling, exclusively in fat storage, inhibits the regeneration-specific response in both the fat storage and the regenerating tissue in Drosophila. Furthermore, in uninjured axolotls we found sex-specific lipid profiles in both storage and circulation, while in regenerating animals these differences subside. The regenerating limb presents a unique sterol profile, albeit with no sex differences. We postulate that regeneration triggers a systemic response, where organs storing lipids play a significant role in the regulation of systemic lipid traffic. Second, that this response may be an active and well-regulated mechanism, as observed when homeostatic sex-differences disappear in regenerating salamanders.
在再生组织中，膜的合成与重塑依赖于脂质周转和运输。我们的研究探讨了黑腹果蝇肠道再生和墨西哥钝口螈肢体再生过程中的脂质适应性。我们发现在不同部位（运输、储存器官和再生组织）的脂质谱存在变化。我们证明，仅在脂肪储存部位减弱胰岛素信号传导，会抑制果蝇脂肪储存部位和再生组织中的再生特异性反应。此外，在未受伤的蝾螈中，我们发现储存和循环中的脂质谱存在性别差异，而在再生动物中这些差异消失。再生肢体呈现出独特的固醇谱，尽管没有性别差异。我们推测，再生会引发一种全身反应，其中储存脂质的器官在全身脂质运输的调节中发挥重要作用。其次，这种反应可能是一种活跃且调节良好的机制，就像在再生蝾螈中稳态性别差异消失时所观察到的那样。
