Nuclear Institute for Agriculture and Biology College, Pakistan Institute of Engineering and Applied Sciences (NIAB-C, PIEAS), Jhang Road, Faisalabad, 38000, Pakistan.
BMC Plant Biol. 2024 Oct 30;24(1):1028. doi: 10.1186/s12870-024-05733-x.
Weed control in chickpea (Cicer arietinum L.) is challenging due to narrow genetic base of available germplasm and limited herbicide options. In this view, present research was focused on induced mutagenesis in chickpea for development of herbicide (glyphosate) tolerant mutants and subsequent screening under field conditions. Further, objective was to analyze the defence response and biochemical adjustments in selected glyphosate tolerant chickpea mutants. Initially, 376 chickpea mutants (M populations developed through EMS and gamma rays) were screened for glyphosate tolerance under filed conditions and scored on a 1 to 5 scale based on plant injury related traits. Among tested mutants, 40 were found highly tolerant (score = 5), 32 as tolerant (score = 4) and 20 as highly sensitive (score = 1) to glyphosate. Chickpea mutants with variable glyphosate tolerance also differed significantly (Tukey test, p < 0.05) in leaf biochemical profiles. For instant, lowest total oxidant status (4175.µM/g f. wt.) was detected in glyphosate tolerant mutant developed from desi chickpea genotype "D3009" using 0.3% EMS and in highly tolerant mutant (1775. µM/g f. wt.) developed from kabuli genotype "K709" using 0.2% EMS. In general, highly tolerant chickpea mutants exhibited highest antioxidant potential (SOD, POD, CAT, TAC) that contributed in glyphosate tolerance. Desi i.e. D1M1HT-2 and Kabuli i.e. KM3HT-2 type mutants with highest seed yield had maximum catalase activity (4200 Units/g f. wt and 540 Units/g f. wt.). Mutants developed from desi type genotypes were comparably superior to mutants derive from Kabuli in terms of herbicide tolerance.
由于现有种质资源遗传基础狭窄和除草剂选择有限，鹰嘴豆中的杂草控制具有挑战性。有鉴于此，本研究专注于鹰嘴豆的诱导突变，以开发对除草剂（草甘膦）具有耐受性的突变体，并随后在田间条件下进行筛选。此外，本研究的目的是分析选定的对草甘膦具有耐受性的鹰嘴豆突变体的防御反应和生化调节。最初，在田间条件下对 376 个鹰嘴豆突变体（通过 EMS 和伽马射线开发的 M 群体）进行了草甘膦耐受性筛选，并根据与植物损伤相关的特征对其进行了 1 到 5 的评分。在测试的突变体中，有 40 个被认为对草甘膦高度耐受（评分=5），32 个被认为耐受（评分=4），20 个对草甘膦高度敏感（评分=1）。具有不同草甘膦耐受性的鹰嘴豆突变体在叶片生化特征上也存在显著差异（Tukey 检验，p<0.05）。例如，用 0.3%EMS 从印度鹰嘴豆品种“D3009”中开发的耐草甘膦突变体的总氧化剂状态最低（4175.µM/g f.wt.），而用 0.2%EMS 从卡布里鹰嘴豆品种“K709”中开发的高度耐受突变体（1775.µM/g f.wt.）的总氧化剂状态最低。一般来说，高度耐受的鹰嘴豆突变体表现出最高的抗氧化潜力（SOD、POD、CAT、TAC），这有助于其对草甘膦的耐受性。具有最高种子产量的 Desi 型即 D1M1HT-2 和 Kabuli 型即 KM3HT-2 突变体具有最高的过氧化氢酶活性（4200 Units/g f.wt.和 540 Units/g f.wt.）。在耐除草剂方面，源自 Desi 型基因型的突变体与源自 Kabuli 的突变体相比具有相当的优势。
