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联合代谢组学和转录组学分析揭示了冬虫夏草水分胁迫的机制。

Combined metabolome and transcriptome analysis reveal the mechanism of water stress in Ophiocordyceps sinensis.

机构信息

School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu, P. R. China.

出版信息

BMC Genomics. 2024 Oct 29;25(1):1014. doi: 10.1186/s12864-024-10785-2.

DOI:10.1186/s12864-024-10785-2
PMID:39472792
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11523607/
Abstract

BACKGROUND

Ophiocordyceps sinensis (O. sinensis) is the dominant bacterium in the asexual stage of Chinese cordyceps, and its growth usually suffers from water stress. Thus, simulating its ecological growth environment is crucial for artificial cultivation. This study aimed to reveal the mechanism underlying the water stress tolerance of Ophiocordyceps sinensis (O. sinensis) by combining metabolomic and transcriptome analyses to identify crucial pathways related to differentially expressed genes (DEGs) and metabolites (DEMs) involved in the response to water stress.

RESULTS

Gene coexpression analysis revealed that many genes related to 'betalain biosynthesis', 'tyrosine metabolism', 'linoleic acid metabolism', 'fructose and mannose metabolism', and 'starch and sucrose metabolism' were highly upregulated after 20d-water stress. Metabolomic analysis revealed that many metabolites regulated by these genes in these metabolic pathways were markedly decreased. On the one hand, we surmised that carbohydrate metabolism and the β-oxidation pathway worked cooperatively to generate enough acyl-CoA and then entered the TCA cycle to provide energy when exposed to water stress. On the other hand, the betalain biosynthesis and tyrosine metabolism pathway might play crucial roles in response to water stress in O. sinensis by enhancing cell osmotic potential and producing osmoregulatory substances (betaine) and antioxidant pigments (eumelanin).

CONCLUSIONS

Overall, our findings provide important information for further exploration of the mechanism underlying the water stress tolerance of O. sinensis for the industrialization of artificial cultivation of Chinese cordyceps.

摘要

背景

冬虫夏草（Ophiocordyceps sinensis）是中国被毛孢无性阶段的优势菌，其生长通常受到水分胁迫的影响。因此，模拟其生态生长环境对于人工栽培至关重要。本研究旨在通过代谢组学和转录组学分析相结合，揭示冬虫夏草对水分胁迫的耐受机制，鉴定与差异表达基因（DEGs）和代谢物（DEMs）相关的关键途径，这些基因和代谢物参与了对水分胁迫的响应。

结果

基因共表达分析表明，在 20d 水分胁迫后，许多与“甜菜碱生物合成”、“酪氨酸代谢”、“亚油酸代谢”、“果糖和甘露糖代谢”和“淀粉和蔗糖代谢”相关的基因高度上调。代谢组学分析表明，这些代谢途径中受这些基因调节的许多代谢物明显减少。一方面，我们推测当暴露在水分胁迫下时，碳水化合物代谢和β-氧化途径协同作用，产生足够的酰基辅酶 A，然后进入 TCA 循环提供能量。另一方面，甜菜碱生物合成和酪氨酸代谢途径可能通过增强细胞渗透压和产生渗透调节物质（甜菜碱）和抗氧化色素（真黑素），在冬虫夏草对水分胁迫的响应中发挥关键作用。

结论

总体而言，我们的研究结果为进一步探索冬虫夏草水分胁迫耐受机制提供了重要信息，为中国被毛孢的人工栽培产业化提供了依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/11523607/306f9b906cb5/12864_2024_10785_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/11523607/ef99e45024aa/12864_2024_10785_Fig2_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/11523607/a00d5e423d17/12864_2024_10785_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/11523607/7e4f911f6dee/12864_2024_10785_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/11523607/edc857f8636e/12864_2024_10785_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/11523607/47bc7ef38d2b/12864_2024_10785_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/11523607/306f9b906cb5/12864_2024_10785_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/11523607/ef99e45024aa/12864_2024_10785_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/11523607/d99c6b86f86b/12864_2024_10785_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/11523607/a9cba54574d3/12864_2024_10785_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/11523607/a00d5e423d17/12864_2024_10785_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/11523607/7e4f911f6dee/12864_2024_10785_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/11523607/edc857f8636e/12864_2024_10785_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/11523607/47bc7ef38d2b/12864_2024_10785_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/11523607/306f9b906cb5/12864_2024_10785_Fig9_HTML.jpg

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