The State Key Laboratory of Southwest Karst Mountain Biodiversity Conservation of Forestry Administration, School of life sciences, Guizhou Normal University, Guiyang 550025, China; The Key Laboratory of Plant Physiology and Development in Guizhou Province, School of life sciences, Guizhou Normal University, Guiyang 550025, China.
The State Key Laboratory of Southwest Karst Mountain Biodiversity Conservation of Forestry Administration, School of life sciences, Guizhou Normal University, Guiyang 550025, China.
Food Res Int. 2023 Jan;163:112275. doi: 10.1016/j.foodres.2022.112275. Epub 2022 Nov 29.
Ophiocordyceps sinensis is a traditional medicinal fungus endemic to the alpine and high-altitude areas of the Qinghai-Tibet plateau. The scarcity of the wild resource has led to increased attention to artificially cultivated O. sinensis. However, little is known about the metabolic differences and the regulatory mechanisms between cultivated and wild O. sinensis. This study exploited untargeted metabolomics and transcriptomics to uncover the differences in accumulated metabolites and expressed genes between wild and cultivated O. sinensis. Metabolomics results revealed that 368 differentially accumulated metabolites were mainly enriched in biosynthesis of amino acids, biosynthesis of plant secondary metabolites and purine nucleotide metabolism. Cultivated O. sinensis contained more amino acids and derivatives, carbohydrates and derivatives, and phenolic acids than wild O. sinensis, whereas the contents of most nucleosides and nucleotides in wild O. sinensis were significantly higher than in cultivated O. sinensis. Transcriptome analysis indicated that 4430 annotated differentially expressed genes were identified between two types. Integrated metabolomics and transcriptomics analyses suggested that IMPDH, AK, ADSS, guaA and GUK genes might be related to the synthesis of purine nucleotides and nucleosides. Our findings will provide a new insight into the molecular basis of metabolic variations of this medicinal fungus.
冬虫夏草是一种传统的药用真菌，仅分布于青藏高原的高寒地区。由于野生资源稀缺，人们对人工栽培的冬虫夏草越来越关注。然而，关于人工栽培与野生冬虫夏草之间的代谢差异及其调控机制，人们知之甚少。本研究利用非靶向代谢组学和转录组学技术，揭示了野生和人工栽培冬虫夏草之间在积累代谢物和表达基因方面的差异。代谢组学结果表明，368 种差异积累代谢物主要富集在氨基酸生物合成、植物次生代谢物生物合成和嘌呤核苷酸代谢途径中。与野生冬虫夏草相比，人工栽培冬虫夏草中含有更多的氨基酸及其衍生物、碳水化合物及其衍生物和酚酸，而野生冬虫夏草中大多数核苷和核苷酸的含量明显高于人工栽培冬虫夏草。转录组分析表明，两种类型之间共鉴定到 4430 个注释差异表达基因。代谢组学和转录组学的综合分析表明，IMP 脱氢酶（IMPDH）、腺嘌呤激酶（AK）、腺苷琥珀酸合成酶（ADSS）、鸟嘌呤核苷酸酶（guaA）和葡萄糖激酶（GUK）基因可能与嘌呤核苷酸和核苷的合成有关。本研究结果将为深入了解该药用真菌代谢变化的分子基础提供新的见解。
